CLC number:
On-line Access: 2022-07-01
Received: 2021-09-17
Revision Accepted: 2022-03-03
Crosschecked: 2022-04-18
Cited: 0
Clicked: 776
Pengtao Wang, Xiaofang Wang, Bo Wang, Xian Li, Zhengsong Xie, Jie Chen, Tasuku Honjo & Xiaolin Tu. 3D printing of osteocytic Dll4 integrated with PCL for cell fate determination towards osteoblasts in vitro[J]. Journal of Zhejiang University Science D, 2022, 5(3): 497-511.
@article{title="3D printing of osteocytic Dll4 integrated with PCL for cell fate
determination towards osteoblasts in vitro",
author="Pengtao Wang, Xiaofang Wang, Bo Wang, Xian Li, Zhengsong Xie, Jie Chen, Tasuku Honjo & Xiaolin Tu",
journal="Journal of Zhejiang University Science D",
volume="5",
number="3",
pages="497-511",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00196-1"
}
%0 Journal Article
%T 3D printing of osteocytic Dll4 integrated with PCL for cell fate
determination towards osteoblasts in vitro
%A Pengtao Wang
%A Xiaofang Wang
%A Bo Wang
%A Xian Li
%A Zhengsong Xie
%A Jie Chen
%A Tasuku Honjo & Xiaolin Tu
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 3
%P 497-511
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00196-1
TY - JOUR
T1 - 3D printing of osteocytic Dll4 integrated with PCL for cell fate
determination towards osteoblasts in vitro
A1 - Pengtao Wang
A1 - Xiaofang Wang
A1 - Bo Wang
A1 - Xian Li
A1 - Zhengsong Xie
A1 - Jie Chen
A1 - Tasuku Honjo & Xiaolin Tu
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 3
SP - 497
EP - 511
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00196-1
Abstract: Since 3D printed hard materials could match the shape of bone, cell survival and fate determination towards osteoblasts in
such materials have become a popular research target. In this study, a scaffold of hard material for 3D fabrication was designed
to regulate developmental signal (Notch) transduction guiding osteoblast differentiation. We established a polycaprolactone
(PCL) and cell-integrated 3D printing system (PCI3D) to reciprocally print the beams of PCL and cell-laden hydrogel for
a module. This PCI3D module holds good cell viability of over 87%, whereas cells show about sixfold proliferation in a
7-day culture. The osteocytic MLO-Y4 was engineered to overexpress Notch ligand Dll4, making up 25% after mixing
with 75% stromal cells in the PCI3D module. osteocytic Dll4, unlike other delta-like family members such as Dll1 or Dll3,
promotes osteoblast differentiation and the mineralization of primary mouse and a cell line of bone marrow stromal cells when
cultured in a PCI3D module for up to 28 days. Mechanistically, osteocytic Dll4 could not promote osteogenic differentiation
of the primary bone marrow stromal cells (BMSCs) after conditional deletion of the Notch transcription factor RBPjκ by
Cre recombinase. These data indicate that osteocytic Dll4 activates RBPjκ-dependent canonical notch signaling in BMSCs
for their oriented differentiation towards osteoblasts. Additionally, osteocytic Dll4 holds a great potential for angiogenesis in
human umbilical vein endothelial cells within modules. Our study reveals that osteocytic Dll4 could be the osteogenic niche
determining cell fate towards osteoblasts. This will open a new avenue to overcome the current limitation of poor cell viability
and low bioactivity of traditional orthopedic implants.
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