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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment
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Weiqin Sheng, Ying Li, Chunlian Qin, Zhonghai Zhang, Yuxiang Pan, Zhicheng Tong, Chong Teng, Xinwei Wei. Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment[J]. Journal of Zhejiang University Science , , (): .
@article{title="Integrated nanoporous electroporation and sensing electrode array for total
dynamic time-domain cardiomyocyte membrane resealing assessment",
author="Weiqin Sheng, Ying Li, Chunlian Qin, Zhonghai Zhang, Yuxiang Pan, Zhicheng Tong, Chong Teng, Xinwei Wei",
journal="Journal of Zhejiang University Science ",
volume="",
number="",
pages="",
year="",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00308-z"
}
%0 Journal Article
%T Integrated nanoporous electroporation and sensing electrode array for total
dynamic time-domain cardiomyocyte membrane resealing assessment
%A Weiqin Sheng
%A Ying Li
%A Chunlian Qin
%A Zhonghai Zhang
%A Yuxiang Pan
%A Zhicheng Tong
%A Chong Teng
%A Xinwei Wei
%J Journal of Zhejiang University SCIENCE
%V
%N
%P
%@ 1869-1951
%D
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00308-z
TY - JOUR
T1 - Integrated nanoporous electroporation and sensing electrode array for total
dynamic time-domain cardiomyocyte membrane resealing assessment
A1 - Weiqin Sheng
A1 - Ying Li
A1 - Chunlian Qin
A1 - Zhonghai Zhang
A1 - Yuxiang Pan
A1 - Zhicheng Tong
A1 - Chong Teng
A1 - Xinwei Wei
J0 - Journal of Zhejiang University Science
VL -
IS -
SP -
EP -
%@ 1869-1951
Y1 -
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00308-z
Abstract: Intracellular electrophysiological research is vital for biological and medical research.
Traditional planar microelectrode arrays (MEAs) have disadvantages in recording
intracellular action potentials due to the loose cellelectrode interface. To investigate
intracellular electrophysiological signals with high sensitivity, electroporation was used
to obtain intracellular recordings. In this study, a biosensing system based on a nanoporous electrode array (NPEA) integrating electrical perforation and signal
acquisition was established to dynamically and sensitively record the intracellular
potential of cardiomyocytes over a long period of time. Moreover, nanoporous
electrodes can induce the protrusion of cell membranes and enhance cellelectrode
interfacial coupling, thereby facilitating effective electroporation. Electrophysiological
signals over the entire recording process can be quantitatively and segmentally analyzed
according to the signal changes, which can equivalently reflect the dynamic evolution
of the electroporated cardiomyocyte membrane. We believe that the low-cost and highperformance nanoporous biosensing platform suggested in this study can dynamically
record intracellular action potential, evaluate cardiomyocyte electroporation, and
provide a new strategy for investigating cardiology pharmacological science.
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