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A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells
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Xinyi LIANG1,2, Changwei QIN1,2, Kaiqiang LI3, An REN1,2, Jiarui HU1,2, Weikang LV1,2, Lunan KE1,2, Zhen WANG3, Mengfei YU4, Xiuxiu JIANG5, Huayong YANG1,2, Xiaobin XU6, Liang MA1,2. A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells[J]. Journal of Zhejiang University Science A, 1998, -1(-1): .
@article{title="A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells",
author="Xinyi LIANG1,2, Changwei QIN1,2, Kaiqiang LI3, An REN1,2, Jiarui HU1,2, Weikang LV1,2, Lunan KE1,2, Zhen WANG3, Mengfei YU4, Xiuxiu JIANG5, Huayong YANG1,2, Xiaobin XU6, Liang MA1,2",
journal="Journal of Zhejiang University Science A",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A2500379"
}
%0 Journal Article
%T A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells
%A Xinyi LIANG1
%A 2
%A Changwei QIN1
%A 2
%A Kaiqiang LI3
%A An REN1
%A 2
%A Jiarui HU1
%A 2
%A Weikang LV1
%A 2
%A Lunan KE1
%A 2
%A Zhen WANG3
%A Mengfei YU4
%A Xiuxiu JIANG5
%A Huayong YANG1
%A 2
%A Xiaobin XU6
%A Liang MA1
%A 2
%J Journal of Zhejiang University SCIENCE A
%V -1
%N -1
%P
%@ 1673-565X
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A2500379
TY - JOUR
T1 - A two-photon polymerization based microfluidic biochip incorporating a herringbone microchannel and deterministic lateral displacement design for efficient capture of circulating tumor cells
A1 - Xinyi LIANG1
A1 - 2
A1 - Changwei QIN1
A1 - 2
A1 - Kaiqiang LI3
A1 - An REN1
A1 - 2
A1 - Jiarui HU1
A1 - 2
A1 - Weikang LV1
A1 - 2
A1 - Lunan KE1
A1 - 2
A1 - Zhen WANG3
A1 - Mengfei YU4
A1 - Xiuxiu JIANG5
A1 - Huayong YANG1
A1 - 2
A1 - Xiaobin XU6
A1 - Liang MA1
A1 - 2
J0 - Journal of Zhejiang University Science A
VL - -1
IS - -1
SP -
EP -
%@ 1673-565X
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A2500379
Abstract: circulating tumor cells (CTCs) are cells that become detached from a primary tumor and enter the vascular or lymphatic system. These cells contain nearly the entire genetic information of the primary tumor. Enrichment and detection of CTCs play a crucial role in prognostications and risk assessments of tumor metastasis and recurrence, evaluation of efficacy and potential medications for precision tumor therapy, and detection of dynamic biomarkers during tumor treatment. Current methods of CTC capture often face the challenge of balancing capture rate and purity. To address these issues, we propose a microfluidic biochip based on the principle of immunoaffinity, which incorporates a herringbone microchannel and deterministic lateral displacement (DLD) technology for the capture of CTCs. By manipulating the internal structural design of the microfluidic chip, we optimized the flow field within the chip, thereby enhancing the contact frequency between cells and aptamers, and ultimately improving the capture rate. The proposed chip demonstrated a capture efficiency of approximately 91.87% for human breast cancer cells (MCF7), with a release rate of 77.5%. The relative viability of the released cells was approximately 94.08%. Notably, the specificity of the aptamers towards tumor cell surface antigens enables high-purity capture. Additionally, the use of DNA enzymes to digest aptamers facilitates the release of high-activity CTCs, offering a method to simultaneously achieve high capture rate, purity, and activity enrichment.
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