CLC number: R94; O657.7
On-line Access:
Received: 2009-08-28
Revision Accepted: 2009-10-26
Crosschecked: 2009-11-03
Cited: 3
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Yan SHEN, Sheng-jie XU, Shi-chun WANG, Jia-sheng TU. Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography[J]. Journal of Zhejiang University Science B, 2009, 10(12): 877-882.
@article{title="Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography",
author="Yan SHEN, Sheng-jie XU, Shi-chun WANG, Jia-sheng TU",
journal="Journal of Zhejiang University Science B",
volume="10",
number="12",
pages="877-882",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920229"
}
%0 Journal Article
%T Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography
%A Yan SHEN
%A Sheng-jie XU
%A Shi-chun WANG
%A Jia-sheng TU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 12
%P 877-882
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920229
TY - JOUR
T1 - Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography
A1 - Yan SHEN
A1 - Sheng-jie XU
A1 - Shi-chun WANG
A1 - Jia-sheng TU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 12
SP - 877
EP - 882
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920229
Abstract: A high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in azithromycin viscous ophthalmic drops. A Venusil-XBP(L)-C18 (150 mm×4.6 mm, 5 μm) column was used at 50 °C. The mobile phase consisted of a mixture of methanol-potassium phosphate (16:5, v/v). Two sample preparation methods were compared. The results suggested that, compared with an extraction procedure, a deproteinization procedure was much quicker and more convenient. Using the deproteinization procedure for sample preparation, calibration curves were linear in the range 5.0~50 μg/ml. The within-day and inter-day coefficients of variation were less than 10%. The average recoveries were determined as 96.70%, 98.52%, and 97.96% at concentrations of 10.0, 30.0, and 50.0 μg/ml, respectively. Variability in precision did not exceed 5%. In conclusion, this HPLC method using a simple sample treatment procedure appears suitable for monitoring BKC content in azithromycin viscous ophthalmic drops.
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