Full Text:
<3576>
CLC number: R71
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2011-12-08
Cited: 5
Clicked: 8699
Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
| ||||||||||||||
Abstract: To evaluate the effects of sperm with different parameters and sources on the outcomes of sperm injection (ICSI)%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation will require further study using a larger sample size.
[1]Antinori, M., Licata, E., Dani, G., Cerusico, F., Versaci, C., d′Angelo, D., Antinori, S., 2008. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod. Biomed. Online, 16(6):835-841.
[2]Aoki, V.W., Wilcox, A.L., Thorp, C., Hamilton, B.D., Carrell, D.T., 2004. Improved in vitro fertilization embryo quality and pregnancy rates with intracytoplasmic sperm injection of sperm from fresh testicular biopsy samples vs. frozen biopsy samples. Fertil. Steril., 82(6):1532-1535.
[3]Bonduelle, M., Camus, M., de Vos, A., Staessen, C., Tournaye, H., van Assche, E., Verheyen, G., Devroey, P., Liebaers, I., van Steirteghem, A., 1999. Seven years of intracytoplasmic sperm injection and follow-up of 1987 subsequent children. Hum. Reprod., 14(s1):243-264.
[4]Bonduelle, M., van Assche, E., Joris, H., Keymolen, K., Devroey, P., van Steirteghem, A., Liebaers, I., 2002. Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters. Hum. Reprod., 17(10):2600-2614.
[5]Braude, P., Bolton, V., Moore, S., 1988. Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature, 332(6163):459-461.
[6]Bukulmez, O., Yucel, A., Yarali, H., Bildirici, I., Gurgan, T., 2001. The origin of spermatozoa does not affect intracytoplasmic sperm injection outcome. Eur. J. Obstet. Gynecol. Reprod. Biol., 94(2):250-255.
[7]Chatterjee, S., Gagnon, C., 2001. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol. Reprod. Dev., 59(4):451-458.
[8]Chen, S.U., Ho, H.N., Chen, H.F., Huang, S.C., Lee, T.Y., Yang, Y.S., 1996. Pregnancy achieved by intracytoplasmic sperm injection using cryopreserved semen from a man with testicular cancer. Hum. Reprod., 11(12):2645-2647.
[9]Chohan, K.R., Griffin, J.T., Carrell, D.T., 2004. Evaluation of chromatin integrity in human sperm using acridine orange staining with different fixatives and after cryopreservation. Andrologia, 36(5):321-326.
[10]Cummins, J.M., Pember, S.M., Jequier, A.M., Yovich, J.L., Hartmann, P.E., 1991. A test of the human sperm acrosome reaction following ionophore challenge: relationship to fertility and other seminal parameters. J. Androl., 12(2):98-103.
[11]Derijck, A.A., van der Heijden, G.W., Ramos, L., Giele, M., Kremer, J.A., de Boer, P., 2007. Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Hum. Reprod., 22(9):2368-2376.
[12]Devroey, P., Liu, J., Nagy, A., Tournaye, H., Silber, S.J., van Steirteghem, A.C., 1994. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil. Steril., 62(3):639-641.
[13]Dozortsev, D., Neme, R., Diamond, M.P., Abdelmassih, S., Abdelmassih, V., Oliveira, F., Abdelmassih, R., 2006. Embryos generated using testicular spermatozoa have higher developmental potential than those obtained using epididymal spermatozoa in men with obstructive azoospermia. Fertil. Steril., 86(3):606-611.
[14]Dubey, A.K., Penzias, A.S., Emmi, A.E., Layman, L.C., Reindollar, R.H., Ducibella, T., 1997. Failed fertilization after intracytoplasmic sperm injection: the extent of paternal and maternal chromatin decondensation. Fertil. Steril., 68(4):714-717.
[15]Dubey, A.K., Penzias, A.S., Reindollar, R.H., Ducibella, T., 1998. Technical and physiological aspects associated with the lower fertilization following intra cytoplasmic sperm injection (ICSI) in human. Theriogenology, 49(1):33-41.
[16]Fedder, J., Gabrielsen, A., Humaidan, P., Erb, K., Ernst, E., Loft, A., 2007. Malformation rate and sex ratio in 412 children conceived with epididymal or testicular sperm. Hum. Reprod., 22(4):1080-1085.
[17]Fenichel, P., Donzeau, M., Farahifar, D., Basteris, B., Ayraud, N., His, B.L., 1991. Dynamics of human sperm acrosome reaction: relation with in vitro fertilization. Fertil. Steril., 55(5):994-999.
[18]FIVNAT, de Mouzon, J., Levy, R., Mourouvin, Z., Belaisch-Allart, J., Bachelot, A., Royère, D., 2007. Semen characteristics and quality of the conceptus in fertilization in vitro. Gynecol. Obstet. Fertil., 35(3):216-223 (in French).
[19]Flaherty, S.P., Payne, D., Swann, N.J., Mattews, C.D., 1995. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum. Reprod., 10(10):2623-2629.
[20]Giraud, M.N., Motta, C., Boucher, D., Grizard, G., 2000. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Hum. Reprod., 15(10):2160-2164.
[21]Göker, E.N., Sendag, F., Levi, R., Sendag, H., Tavmergen, E., 2002. Comparison of the ICSI outcome of ejaculated sperm with normal, abnormal parameters and testicular sperm. Eur. J. Obstet. Gynecol. Reprod. Biol., 104(2):129-136.
[22]Heytens, E., Parrington, J., Coward, K., Young, C., Lambrecht, S., Yoon, S.Y., Fissore, R.A., Hamer, R., Deane, C.M., Ruas, M., et al., 2009. Reduced amounts and abnormal forms of phospholipase C zeta (PLCζ) in spermatozoa from infertile men. Hum. Reprod., 24(10):2417-2428.
[23]Holt, W.V., North, R.D., 1991. Cryopreservation, actin localization and thermotropic phase transitions in ram spermatozoa. J. Reprod. Fertil., 91(2):451-461.
[24]Holt, W.V., Head, M.F., North, R.D., 1992. Freeze-induced membrane damage in ram spermatozoa is manifested after thawing: observations with experimental cryomicroscopy. Biol. Reprod., 46(6):1086-1094.
[25]Huszar, G., Jakab, A., Sakkas, D., Ozenci, C.C., Cayli, S., Delpiano, E., Ozkavukcu, S., 2007. Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod. Biomed. Online, 14(5):650-663.
[26]Kobayashi, H., Sato, A., Otsu, E., Hiura, H., Tomatsu, C., Utsunomiya, T., Sasaki, H., Yaegashi, N., Arima, T., 2007. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum. Mol. Genet., 16(21):2542-2551.
[27]Kolettis, P.N., Sharma, R.K., Pasqualotto, F.F., Nelson, D., Thomas, A.J., Agarwal, A., 1999. Effect of seminal oxidative stress on fertility after vasectomy reversal. Fertil. Steril., 71(2):249-255.
[28]Lin, Y.M., Hsu, C.C., Kuo, T.C., Lin, J.S., Wang, S.T., Huang, K.E., 2000. Percutaneous epididymal sperm aspiration versus microsurgical epididymal sperm aspiration for irreparable obstructive azoospermia—experience with 100 cases. J. Formos. Med. Assoc., 99(6):459-465.
[29]Liu, C.H., Tsao, H.M., Cheng, T.C., Wu, H.M., Huang, C.C., Chen, C.I., Lin, D.P., Lee, M.S., 2004. DNA fragmentation, mitochondrial dysfunction and chromosomal aneuploidy in the spermatozoa of oligoasthenoteratozoospermic males. J. Assist. Reprod. Genet., 21(4):119-126.
[30]Loutradi, K.E., Tarlatzis, B.C., Goulis, D.G., Zepiridis, L., Pagou, T., Chatziioannou, E., Grimbizis, G.F., Papadimas, I., Bontis, I., 2006. The effects of sperm quality on embryo development after intracytoplasmic sperm injection. J. Assist. Reprod. Genet., 23(2):69-74.
[31]Ludwig, M., Katalinic, A., 2003. Pregnancy course and health of children born after ICSI depending on parameters of male factor infertility. Hum. Reprod., 18(2):351-357.
[32]Miller, J.E., Smith, T.T., 2001. The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro. Hum. Reprod., 16(5):918-924.
[33]Mortimer, D., 2000. Sperm preparation methods. J. Androl., 21(3):357-366.
[34]Naru, T., Sulaiman, M.N., Kidwai, A., Ather, M.H., Waqar, S., Virk, S., Rizvi, J.H., 2008. Intracytoplasmic sperm injection outcome using ejaculated sperm and retrieved sperm in azoospermic men. Urol. J., 5(2):106-110.
[35]Nilsson, S., Waldenström, U., Engström, A.B., Hellberg, D., 2007. Single blastocyst transfer after ICSI from ejaculate spermatozoa, percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE). J. Assist. Reprod. Genet., 24(5):167-171.
[36]Oliveira, J.B., Petersen, C.G., Massaro, F.C., Baruffi, R.L., Mauri, A.L., Silva, L.F., Ricci, J., Franco, J.G., 2010. Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles. Reprod. Biol. Endocrinol., 8(1):56.
[37]Palermo, G., Joris, H., Devroey, P., van Steirteghem, A.C., 1992. Pregnancies after intracytoplasmic injection of a single permatozoon into an oocyte. Lancet, 340(8810):17-18.
[38]Parmegiani, L., Cognigni, G.E., Bernardi, S., Troilo, E., Ciampaglia, W., Filicori, M., 2010. “Physiologic ICSI”: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil. Steril., 93(2):598-604.
[39]Pegg, D.E., 2002. The history and principles of cryopreservation. Semin. Reprod. Med., 20(1):5-13.
[40]Perreault, S.D., 1992. Chromatin remodeling in mammalian zygotes. Mutat. Res., 296(1-2):43-55.
[41]Perreault, S.D., Barbee, R.R., Slott, V.L., 1988. Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. Dev. Biol., 125(1):181-186.
[42]Ramos, L., Kleingeld, P., Meuleman, E., van Kooy, R., Kremer, J., Braat, D., Wetzels, A., 2002. Assessment of DNA fragmentation of spermatozoa that were surgically retrieved from men with obstructive azoospermia. Fertil. Steril., 77(2):233-237.
[43]Redgment, C.J., Yang, D., Tsirigotis, M., Yazdani, N., al Shawaf, T., Craft, I.L., 1994. Experience with assisted fertilization in severe male factor infertility and unexplained failed fertilization in vitro. Hum. Reprod., 9(4):680-683.
[44]Rimm, A.A., Katayama, A.C., Diaz, M., Katayama, K.P., 2004. A meta-analysis of controlled studies comparing major malformation rates in IVF and ICSI infants with naturally conceived children. J. Assist. Reprod. Genet., 21(12):437-443.
[45]Royere, D., Barthelemy, C., Hamamah, S., Lansac, J., 1996. Cryopreservation of spermatozoa: a 1996 review. Hum. Reprod. Update, 2(6):553-559.
[46]Sakkas, D., Mariethoz, E., Manicardi, G., Bizzaro, D., Bianchi, P.G., Bianchi, U., 1999. Origin of DNA damage in ejaculated human spermatozoa. Rev. Reprod., 4(1):31-37.
[47]Sarkar, N.N., 2007. Intracytoplasmic sperm injection: an assisted reproductive technique and its outcome to overcome infertility. J. Obstet. Gynaecol., 27(4):347-353.
[48]Schatten, G., 1994. The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization. Dev. Biol., 165(2):299-335.
[49]Seli, E., Gardner, D.K., Schoolcraft, W.B., Moffatt, O., Sakkas, D., 2004. Extend of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil. Steril., 82(2):378-383.
[50]Shapiro, R.H., Muller, C.H., Chen, G., Berger, R.E., 1998. Vasectomy reversal associated with increased reactive oxygen species production by seminal fluid leukocytes and sperm. J. Urol., 160(4):1341-1346.
[51]Swann, L., Larman, M.G., Saunders, C.M., Lai, F.A., 2004. The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCζ. Reproduction, 127(4):431-439.
[52]Tarlatzis, B.C., Pados, G., Bontis, J., Lagos, S., Gribizis, G., Spanos, E., Mantalenakis, S., 1993. Ovarian stimulation with buserilin/HMG/HCG: prospective randomized study of short versus long protocol. Hum. Reprod., 8(6):807-812.
[53]Tavalaee, M., Razavi, S., Nasr-Esfahani, M.H., 2009. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil. Steril., 91(4):1119-1126.
[54]Tepla, O., Peknicova, J., Koci, K., Mika, J., Mrazek, M., Elzeinova, F., 2006. Evaluation of reproductive potential after intracytoplasmic sperm injection of varied human semen tested by antiacrosomal antibodies. Fertil. Steril., 86(1):113-120.
[55]Tournaye, H., Devroey, P., Liu, J., Nagy, Z., Lissens, W., van Steirteghem, A., 1994. Microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection: a new effective approach to infertility as a result of congenital bilateral absence of the vas deferens. Fertil. Steril., 61(6):1045-1051.
[56]van Steirteghem, A.C., Nagy, Z., Joris, H., Liu, J., Staessen, C., Smitz, J., Wisanto, A., Devroey, P., 1993. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum. Reprod., 8(7):1061-1066.
[57]Veek, L.L., 1990. The Morphological Assessment of Human Oocytes and Early Conception. In: Keel, B.A., Webster, B.W. (Eds.), Hand-Book of the Laboratory Diagnosis and Treatment of Infertility. CRC Press, Voca Raton, p.353-369.
[58]Verza, S.Jr., Esteves, S.C., 2008. Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection. Int. Braz. J. Urol., 34(1):49-56.
[59]Virro, M.R., Larson-Cook, K.L., Evenson, D.P., 2004. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil. Steril., 81(5):1289-1295.
[60]Watkins, W., Nieto, F., Bourne, H., Wutthiphan, B., Speirs, A., Baker, H.W., 1997. Testicular and epididymal sperm in a microinjection program; methods of retrieval and results. Fertil. Steril., 67(3):527-535.
[61]Wilding, M., Coppola, G., di Matteo, L., Palagiano, A., Fusco, E., Dale, B., 2011. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J. Assist. Reprod. Genet., 28(3):253-262.
[62]WHO, 2010. WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th Ed. World Health Organization Press, Geneva, p.7-114.
[63]Yanagimachi, R., 1994. Mammalian Fertilization. In: Knobil, E., Neil, J.D. (Eds.), Physiology of Reproduction. Raven Press, New York, p.189-317.
Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin. Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation[J]. Journal of Zhejiang University Science B, 2012, 13(1): 1-10.
@article{title="Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation",
author="Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin",
journal="Journal of Zhejiang University Science B",
volume="13",
number="1",
pages="1-10",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100216"
}
%0 Journal Article
%T Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
%A Yue-hong Lu
%A Hui-juan Gao
%A Bai-jia Li
%A Ying-ming Zheng
%A Ying-hui Ye
%A Yu-li Qian
%A Chen-ming Xu
%A He-feng Huang
%A Fan Jin
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 1
%P 1-10
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100216
TY - JOUR
T1 - Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
A1 - Yue-hong Lu
A1 - Hui-juan Gao
A1 - Bai-jia Li
A1 - Ying-ming Zheng
A1 - Ying-hui Ye
A1 - Yu-li Qian
A1 - Chen-ming Xu
A1 - He-feng Huang
A1 - Fan Jin
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 1
SP - 1
EP - 10
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100216
Click to get updates and verify authenticity.
Open peer comments: Debate/Discuss/Question/Opinion
<1>