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Abstract: axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs (miRNAs), and transcription factors (TFs) that influence axon regeneration. However, the regulatory networks involved have not been fully elucidated. In the present study, we analyzed a regulatory network of 51 miRNAs, 27 TFs, and 59 target genes, which is involved in axon regeneration. We identified 359 pairs of feed-forward loops (FFLs), seven important genes (Nap1l1, Arhgef12, Sema6d, Akt3, Trim2, Rab11fip2, and Rps6ka3), six important miRNAs (hsa-miR-204-5p, hsa-miR-124-3p, hsa-miR-26a-5p, hsa-miR-16-5p, hsa-miR-17-5p, and hsa-miR-15b-5p), and eight important TFs (Smada2, Fli1, Wt1, Sp6, Sp3, Smad4, Smad5, and Creb1), which appear to play an important role in axon regeneration. Functional enrichment analysis revealed that axon-associated genes are involved mainly in the regulation of cellular component organization, axonogenesis, and cell morphogenesis during neuronal differentiation. However, these findings need to be validated by further studies.

神经轴突再生过程中微小RNA(miRNA)、转录因子和靶基因的网络功能分析

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[37]List of electronic supplementary materials

[38]Table S1 Information of DEGs between Axon hESC-Neuron and Whole hESC-Neuron

[39]Table S2 Overlapping genes between DEGs and genes related to axon and genes in the same family with overlapping genes (Genes Cluster 1)

[40]Table S3 Mature miRNAs related to human axon

[41]Table S4 Target genes of miRNAs (Genes Cluster 2)

[42]Table S5 TFs interaction with 59 genes (TFs Cluster 2)

[43]Table S6 TFs interaction with 51 miRNAs (TFs Cluster 3)

[44]Table S7 Feed-forward loops in the network

[45]Table S8 Gene ontology enrichment analysis of biological process