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CLC number: R96

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2019-06-13

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yan-chun Hu

https://orcid.org/0000-0002-2998-7879

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.8 P.693-698

http://doi.org/10.1631/jzus.B1800645


Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice


Author(s):  Wei Sun, Chao-rong Zeng, Dong Yue, Yan-chun Hu

Affiliation(s):  Key Laboratory of Animal Disease and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):   hychun114@163.com

Key Words:  Ageratina adenophora, Hepatotoxicity, Mitochondrial dysfunction, ATPase, Mitochondrial DNA


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Wei Sun, Chao-rong Zeng, Dong Yue, Yan-chun Hu. Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice[J]. Journal of Zhejiang University Science B, 2019, 20(8): 693-698.

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Abstract: 
Ageratina adenophora is a noxious plant and it is known to cause acute asthma, diarrhea, depilation, and even death in livestock (Zhu et al., 2007; Wang et al., 2017). A. adenophora grows near roadsides and degraded land worldwide (He et al., 2015b). In the areas where it grows, A. adenophora is an invasive species that inhibits the growth of local plants and causes poisoning in animals that come in contact with it (Nie et al., 2012). In China, these plants can be found in Yunnan, Sichuan, Guizhou, Chongqing, and other southwestern areas (He et al., 2015a) and they have become a dominant species in these local regions. It threatens the native biodiversity and ecosystem in the invaded areas and causes serious economic losses (Wang et al., 2017). It has been reported that A. adenophora can grow in the northeast direction at a speed of 20 km per year in China (Guo et al., 2009). Because of the damage caused by A. adenophora, it ranks among the earliest alien invasive plant species in China (Wang et al., 2017).

紫茎泽兰引起小鼠线粒体功能障碍从而导致肝脏毒性损伤

目的:研究紫茎泽兰造成小鼠肝脏毒性损伤后,肝脏线粒体结构和功能发生变化的情况.
创新点:紫茎泽兰可以导致肝脏细胞发生凋亡和焦亡,从而产生中毒损伤,然而尚未有报道其对线粒体超微结构和功能的改变.本研究通过多种手段解决了这一问题.
方法:将40只小鼠随机分成4组,分别饲喂不同浓度的紫茎泽兰饲料(对照组、100、200和300 g/kg紫茎泽兰添加饲料组).采用苏木精-伊红染色法(H&E)研究肝脏损伤情况,利用透射电子显微技术研究线粒体超微结构改变.同时,结合实时荧光定量聚合酶链反应(qRT-PCR)、流式细胞术和化学分析方法对线粒体DNA拷贝数、肿胀度和三磷酸腺苷(ATP)酶活性的改变进行探究.
结论:紫茎泽兰导致线粒体超微结构的改变,增大了线粒体肿胀度,降低了钠钾ATP酶(Na+K+-ATPase)和钙镁ATP酶(Ca2+Mg2+-ATPase)活力,同时减少了DNA拷贝数,从而引起肝脏损伤.

关键词:紫茎泽兰;肝毒性;线粒体功能障碍;三磷酸腺苷(ATP)酶;线粒体DNA

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[27]Data S1 Materials and methods

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