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Suppl. Mater.: 

CLC number: R614

On-line Access: 2015-09-05

Received: 2015-01-05

Revision Accepted: 2015-04-29

Crosschecked: 2015-08-10

Cited: 5

Clicked: 3525

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Ye Zhang

http://orcid.org/0000-0001-6328-8003

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.9 P.763-771

http://doi.org/10.1631/jzus.B1500006


Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia


Author(s):  Yun-zhi Ling, Wei Ma, Li Yu, Ye Zhang, Qi-sheng Liang

Affiliation(s):  1Department of Anesthesiology, the Second Affiliated Hospital of Anhui Medical University, Anhui 230601, China; more

Corresponding email(s):   zhang_ye011@163.com

Key Words:  Sevoflurane, Cognitive dysfunction, PSD95


Yun-zhi Ling, Wei Ma, Li Yu, Ye Zhang, Qi-sheng Liang. Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia[J]. Journal of Zhejiang University Science B, 2015, 16(9): 763-771.

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author="Yun-zhi Ling, Wei Ma, Li Yu, Ye Zhang, Qi-sheng Liang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="9",
pages="763-771",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500006"
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%T Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia
%A Yun-zhi Ling
%A Wei Ma
%A Li Yu
%A Ye Zhang
%A Qi-sheng Liang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 9
%P 763-771
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500006

TY - JOUR
T1 - Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia
A1 - Yun-zhi Ling
A1 - Wei Ma
A1 - Li Yu
A1 - Ye Zhang
A1 - Qi-sheng Liang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 9
SP - 763
EP - 771
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500006


Abstract: 
Objective: Though sevoflurane has been widely used as an anesthetic in surgery, recent studies have shown that exposure to sevoflurane alone could lead to postoperative cognitive dysfunction (POCD), of which the mechanisms still remain largely unknown. The medial prefrontal cortex (mPFC) is known to be implicated in various cognitive impairments, including working memory and attentional processes. In the present study, we tried to identify dysregulated gene expression in mPFC and investigate the underlying mechanisms involved in POCD. Methods: Behavioral tests, including elevated plus-maze, O-maze, and Y-maze tests, were performed on Wistar rats exposed to sevoflurane. Whole-genome mRNA profiling of mPFC from Wistar rats after exposure to sevoflurane was carried out. Real-time polymerase chain reaction (PCR) was done to verify the differentially expressed genes. Results: Significant impairment of working memory of rats after exposure to sevoflurane was observed. A total of 119 of 7319 detected mRNAs showed significantly different expression between rats with and without sevoflurane exposure (fold change (FC)>2.0, P<0.05, and false discovery rate (FDR)<0.05), among which 74 mRNAs were down-regulated and 45 mRNAs were up-regulated. Postsynaptic density-95 (PSD95, also named DLG4) showed the most significantly decreased expression in mPFC and further investigation indicated that PSD95 expression level was correlated with spatial working memory performance. Conclusions: Our study revealed that PSD95 might be involved in the mechanism of POCD, which could provide clues for preventing POCD in clinical operations.

七氟烷麻醉诱导的认知功能损伤与内测前额叶皮层中PSD95表达量降低有关

目的:临床上接受七氟烷麻醉的病人,术后会出现认知功能损伤。本研究旨在挖掘七氟烷麻醉导致的内侧前额叶皮层中基因表达谱的改变,并探讨术后认知功能改变的机制。
创新点:本研究深入探讨七氟烷麻醉导致认知功能损伤的分子机制,充分利用表达谱和动物行为学实验来揭示可能的分子机制。
方法:采用十字迷宫、O迷宫和水迷宫分析接受不同时间七氟烷处理的Wistar大鼠的行为学特征;同时提取实验大鼠内侧前额叶皮层的mRNA进行表达谱分析(图3),采用实时聚合酶链反应(real-time PCR)对差异表达mRNA进行验证。
结论:内侧前额叶皮层中PSD95表达量降低与七氟烷 麻醉诱导的认知功能损伤有关。

关键词:七氟烷;认知功能损伤;PSD95

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[35]List of electronic supplementary materials

[36]Table S1 Dysregulated mRNAs in in medial prefrontal cortex between experimental rats and normal rats

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