Combinatorial mutagenesis and transcriptomic dissection of a Wickerhamomyces anomalus strain for enhanced d-arabitol production
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Jikun XIA1, Jialin ZHU1, Xudong XU1, Lvyang LIN1, Jianping LIN1,2, Lidan YE1, Mianbin WU1,2. Combinatorial mutagenesis and transcriptomic dissection of a Wickerhamomyces anomalus strain for enhanced d-arabitol production[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Combinatorial mutagenesis and transcriptomic dissection of a Wickerhamomyces anomalus strain for enhanced d-arabitol production",
author="Jikun XIA1, Jialin ZHU1, Xudong XU1, Lvyang LIN1, Jianping LIN1,2, Lidan YE1, Mianbin WU1,2",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2600110"
}
%0 Journal Article
%T Combinatorial mutagenesis and transcriptomic dissection of a Wickerhamomyces anomalus strain for enhanced d-arabitol production
%A Jikun XIA1
%A Jialin ZHU1
%A Xudong XU1
%A Lvyang LIN1
%A Jianping LIN1
%A 2
%A Lidan YE1
%A Mianbin WU1
%A 2
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
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%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2600110
TY - JOUR
T1 - Combinatorial mutagenesis and transcriptomic dissection of a Wickerhamomyces anomalus strain for enhanced d-arabitol production
A1 - Jikun XIA1
A1 - Jialin ZHU1
A1 - Xudong XU1
A1 - Lvyang LIN1
A1 - Jianping LIN1
A1 - 2
A1 - Lidan YE1
A1 - Mianbin WU1
A1 - 2
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP - 0
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2600110
Abstract: Industrial biomanufacturing of d-Arabitol, a high-value sugar alcohol, is currently constrained by the scarcity of robust and high-yielding microbial strains. To address this issue, we isolated a wild-type strain, Wickerhamomyces anomalus K955, and engineered it using a combinatorial mutagenesis strategy that integrated low-energy N+ ion implantation with diethyl sulfate (DES) treatment, coupled with an iodonitrotetrazolium violet (INT)-based high-throughput screening (HTS) method. The resulting mutant, A37, exhibited superior performance, achieving a titer of 108.69 g/L and a yield of 0.31 g/g in shake-flask fermentation. Transcriptomic analysis showed that this hyper-producing phenotype resulted from global metabolic reprogramming: the strain demonstrated enhanced biosynthetic capacity via upregulated ribosome biogenesis, while carbon flux was effectively redirected toward the Pentose Phosphate Pathway (PPP) through synergistic upregulation of key genes, including zwf, gnd, and rpe. In scaled-up fed-batch fermentation, strain A37 produced 220.85 g/L of d-Arabitol with a productivity of 1.28 g/(L·h), representing the highest levels reported to date. These findings provide a superior candidate strain for industrial d-Arabitol production and offer critical insights into the metabolic regulation mechanisms underlying high-efficiency biosynthesis.
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