CLC number:
On-line Access: 2021-04-24
Received: 2020-12-02
Revision Accepted: 2021-03-13
Crosschecked: 0000-00-00
Cited: 0
Clicked: 3391
Shengyu Chen, Fan Jia, Luying Zhao, Fuyu Qiu, Shaohua Jiang, Jian Ji & Guosheng Fu. Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth[J]. Journal of Zhejiang University Science D, 2021, 4(3): 469-478.
@article{title="Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth",
author="Shengyu Chen, Fan Jia, Luying Zhao, Fuyu Qiu, Shaohua Jiang, Jian Ji & Guosheng Fu",
journal="Journal of Zhejiang University Science D",
volume="4",
number="3",
pages="469-478",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00131-w"
}
%0 Journal Article
%T Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth
%A Shengyu Chen
%A Fan Jia
%A Luying Zhao
%A Fuyu Qiu
%A Shaohua Jiang
%A Jian Ji & Guosheng Fu
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 3
%P 469-478
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00131-w
TY - JOUR
T1 - Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth
A1 - Shengyu Chen
A1 - Fan Jia
A1 - Luying Zhao
A1 - Fuyu Qiu
A1 - Shaohua Jiang
A1 - Jian Ji & Guosheng Fu
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 3
SP - 469
EP - 478
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00131-w
Abstract: The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these conventional drugs can lead to the failure of blood vessel reconstruction, leading to potential complications in the long term. To address this issue, an asymmetric membrane was constructed through electro-spinning techniques. The bilayer membrane loaded and effectively released nitric oxide (NO), as hoped, from only one side. Due to the short diffusion distance of NO, it exerted negligible effects on the other side of the membrane, thus allowing selective regulation of different cells on both sides. The released NO boosted the growth of endothelial cells (ECs) over smooth muscle cells (SMCs)—while on the side where NO was absent, SMCs grew into multilayers. The overall structure resembled a native blood vessel, with confluent ECs as the inner layer and layers of SMCs to support it. In addition, the membrane preserved the normal function of ECs, and at the same time did not exacerbate inflammatory responses. By preparing this material type that regulates cell behavior differentially, we describe a new method for its application in the cardiovascular field such as for artificial blood vessels.
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