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On-line Access: 2022-07-01

Received: 2021-11-05

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Bio-Design and Manufacturing  2022 Vol.5 No.3 P.633-639

http://doi.org/10.1007/s42242-022-00189-0


Projection-based 3D bioprinting for hydrogel scaffold manufacturing


Author(s):  Yuan Sun, Kang Yu, Qing Gao & Yong He

Affiliation(s):  State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   yongqin@zju.edu.cn

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Yuan Sun, Kang Yu, Qing Gao & Yong He. Projection-based 3D bioprinting for hydrogel scaffold manufacturing[J]. Journal of Zhejiang University Science D, 2022, 5(3): 633-639.

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Abstract: 

浙江大学贺永等 | 光固化生物3D打印教程之高精度水凝胶支架制造

本Technical Note聚焦光固化生物3D打印之高精度水凝胶支架制造。投影式光固化生物打印(Projection-based 3D bioprinting, PBP)具有精度高、速度快、自支撑等优势,近年来在生物制造领域有越来越多PBP的成功应用案例。然而,完全理解PBP的原理需要光学、高分子、工程、生物学等综合学科的背景知识,初学者熟练掌握PBP的流程也需要经历长时间的试错与经验积累。降低PBP的入门使用门槛,可使更多研究者能从这项技术中受益。


本文给出了一个标准的水凝胶支架打印流程,我们将PBP生物支架打印分为“材料配制”、“支架打印”、“后处理”与“细胞接种”四个工序,给出了标准化的操作方法,整理了很多常见的问题、技巧及背后机制。通过这篇教程,希望能够帮助PBP初学者,降低非工程学科背景使用者在工艺测试上的时间和成本,将PBP作为得力工具加速其研究。

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