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On-line Access: 2022-07-01
Received: 2021-11-05
Revision Accepted: 2022-02-06
Crosschecked: 2022-03-14
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Projection-based 3D bioprinting for hydrogel scaffold manufacturing
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Yuan Sun, Kang Yu, Qing Gao & Yong He. Projection-based 3D bioprinting for hydrogel scaffold manufacturing[J]. Journal of Zhejiang University Science D, 2022, 5(3): 633-639.
@article{title="Projection-based 3D bioprinting for hydrogel scaffold manufacturing",
author="Yuan Sun, Kang Yu, Qing Gao & Yong He",
journal="Journal of Zhejiang University Science D",
volume="5",
number="3",
pages="633-639",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00189-0"
}
%0 Journal Article
%T Projection-based 3D bioprinting for hydrogel scaffold manufacturing
%A Yuan Sun
%A Kang Yu
%A Qing Gao & Yong He
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 3
%P 633-639
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00189-0
TY - JOUR
T1 - Projection-based 3D bioprinting for hydrogel scaffold manufacturing
A1 - Yuan Sun
A1 - Kang Yu
A1 - Qing Gao & Yong He
J0 - Journal of Zhejiang University Science D
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IS - 3
SP - 633
EP - 639
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
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DOI - 10.1007/s42242-022-00189-0
Abstract:
本Technical Note聚焦光固化生物3D打印之高精度水凝胶支架制造。投影式光固化生物打印(Projection-based 3D bioprinting, PBP)具有精度高、速度快、自支撑等优势，近年来在生物制造领域有越来越多PBP的成功应用案例。然而，完全理解PBP的原理需要光学、高分子、工程、生物学等综合学科的背景知识，初学者熟练掌握PBP的流程也需要经历长时间的试错与经验积累。降低PBP的入门使用门槛，可使更多研究者能从这项技术中受益。
本文给出了一个标准的水凝胶支架打印流程，我们将PBP生物支架打印分为“材料配制”、“支架打印”、“后处理”与“细胞接种”四个工序，给出了标准化的操作方法，整理了很多常见的问题、技巧及背后机制。通过这篇教程，希望能够帮助PBP初学者，降低非工程学科背景使用者在工艺测试上的时间和成本，将PBP作为得力工具加速其研究。
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