CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2022-04-04
Cited: 0
Clicked: 1005
Marcin Kotlarz, Ana M. Ferreira, Piergiorgio Gentile, Stephen J. Russell & Kenneth Dalgarno. Droplet-based bioprinting enables the fabrication of cell–hydrogel–microfibre composite tissue precursors[J]. Journal of Zhejiang University Science D, 2022, 5(3): 512-528.
@article{title="Droplet-based bioprinting enables the fabrication
of cell–hydrogel–microfibre composite tissue precursors",
author="Marcin Kotlarz, Ana M. Ferreira, Piergiorgio Gentile, Stephen J. Russell & Kenneth Dalgarno",
journal="Journal of Zhejiang University Science D",
volume="5",
number="3",
pages="512-528",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00192-5"
}
%0 Journal Article
%T Droplet-based bioprinting enables the fabrication
of cell–hydrogel–microfibre composite tissue precursors
%A Marcin Kotlarz
%A Ana M. Ferreira
%A Piergiorgio Gentile
%A Stephen J. Russell & Kenneth Dalgarno
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 3
%P 512-528
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00192-5
TY - JOUR
T1 - Droplet-based bioprinting enables the fabrication
of cell–hydrogel–microfibre composite tissue precursors
A1 - Marcin Kotlarz
A1 - Ana M. Ferreira
A1 - Piergiorgio Gentile
A1 - Stephen J. Russell & Kenneth Dalgarno
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 3
SP - 512
EP - 528
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00192-5
Abstract: Composites offer the option of coupling the individual benefits of their constituents to achieve unique material properties,
which can be of extra value in many tissue engineering applications. Strategies combining hydrogels with fibre-based scaffolds
can create tissue constructs with enhanced biological and structural functionality. However, developing efficient and scalable
approaches to manufacture such composites is challenging. Here, we use a droplet-based bioprinting system called reactive jet
impingement (ReJI) to integrate a cell-laden hydrogel with a microfibrous mesh. This system uses microvalves connected to
different bioink reservoirs and directed to continuously jet bioink droplets at one another in mid-air, where the droplets react
and form a hydrogel that lands on a microfibrous mesh. Cell–hydrogel–fibre composites are produced by embedding human
dermal fibroblasts at two different concentrations (5 × 106 and 30 × 106 cells/mL) in a collagen–alginate–fibrin hydrogel
matrix and bioprinted onto a fibre-based substrate. Our results show that both types of cell–hydrogel–microfibre composite
maintain high cell viability and promote cell–cell and cell–biomaterial interactions. The lower fibroblast density triggers cell
proliferation, whereas the higher fibroblast density facilitates faster cellular organisation and infiltration into the microfibres.
Additionally, the fibrous component of the composite is characterised by high swelling properties and the quick release of
calcium ions. The data indicate that the created composite constructs offer an efficient way to create highly functional tissue
precursors for laminar tissue engineering, particularly for wound healing and skin tissue engineering applications.
Open peer comments: Debate/Discuss/Question/Opinion
<1>