JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.2 P.118-125

Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine

Author(s): Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi
Affiliation(s): School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; more
Corresponding email(s): jintliu@sdu.edu.cn, xirimo2000@yahoo.com
Key Words: Gatifloxacin, Drug residue, Antibody, Carbon fiber electrode, Electrochemical immunoassay

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Abstract: To detect gatifloxacin (GAT) residue in swine urine, an electrochemical immunoassay was established. An indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (ELISA) plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase (HRP) conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator. The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration (IC₅₀) of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin (3.0%), ciprofloxacin (3.0%), and ofloxacin (1.9%) among commonly used (fluoro)quinolones. In conclusion, the immunoassay system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods and food products.

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