Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer
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Qiu-xia Jin, Xin-ran Dong, Jing-meng Chen, Ke Yao, Ya-lin Wu. Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer[J]. Journal of Zhejiang University Science B, 2014, 15(7): 661-669.
@article{title="Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer",
author="Qiu-xia Jin, Xin-ran Dong, Jing-meng Chen, Ke Yao, Ya-lin Wu",
journal="Journal of Zhejiang University Science B",
volume="15",
number="7",
pages="661-669",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300194"
}
%0 Journal Article
%T Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer
%A Qiu-xia Jin
%A Xin-ran Dong
%A Jing-meng Chen
%A Ke Yao
%A Ya-lin Wu
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 7
%P 661-669
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300194
TY - JOUR
T1 - Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer
A1 - Qiu-xia Jin
A1 - Xin-ran Dong
A1 - Jing-meng Chen
A1 - Ke Yao
A1 - Ya-lin Wu
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 7
SP - 661
EP - 669
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300194
Abstract: Gene and drug therapies are being developed to alleviate vision loss in patients with Stargardt’s disease and age-related macular degeneration (AMD). To evaluate the therapeutic effects of these treatments, organic solvents are routinely used to extract and quantify bisretinoid lipofuscin constituents, such as N-retinylidene-N-retinyl-ethanolamine (A2E) and all-trans-retinal dimer (ATR-dimer). By high-performance liquid chromatography (HPLC), we found that A2E and ATR-dimer were both altered by tetrahydrofuran (THF) and chloroform, but were stable in dimethyl sulfoxide (DMSO) or methanol (MeOH). In addition, cyclohexane and ethanol (EtOH) did not alter ATR-dimer, whereas an alteration of A2E occurred in EtOH. On the basis of these findings, we designed processes II–IV, generated by modifications of process I, a routine method to measure bisretinoid compounds in vivo. Extra amounts of either ATR-dimer or A2E in mouse eyecups were released by processes II–IV versus process I. Efforts to clarify the effects of organic solvents on lipofuscin pigments are important because such studies can guide the handling of these fluorophores in related experiments.
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CLC number: R774.5
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-05-07
Cited: 0
Clicked: 11347
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