Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo
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Zhan-huai Wang, Qiong Li, Shu-qin Ruan, Qian Xiao, Yue Liu, Ye-ting Hu, Li-feng Hu, Hai-yan Chen, Shu Zheng, Su-zhan Zhang, Ke-feng Ding. Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo[J]. Journal of Zhejiang University Science B, 2014, 15(8): 701-712.
@article{title="Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo",
author="Zhan-huai Wang, Qiong Li, Shu-qin Ruan, Qian Xiao, Yue Liu, Ye-ting Hu, Li-feng Hu, Hai-yan Chen, Shu Zheng, Su-zhan Zhang, Ke-feng Ding",
journal="Journal of Zhejiang University Science B",
volume="15",
number="8",
pages="701-712",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300306"
}
%0 Journal Article
%T Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo
%A Zhan-huai Wang
%A Qiong Li
%A Shu-qin Ruan
%A Qian Xiao
%A Yue Liu
%A Ye-ting Hu
%A Li-feng Hu
%A Hai-yan Chen
%A Shu Zheng
%A Su-zhan Zhang
%A Ke-feng Ding
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 8
%P 701-712
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300306
TY - JOUR
T1 - Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo
A1 - Zhan-huai Wang
A1 - Qiong Li
A1 - Shu-qin Ruan
A1 - Qian Xiao
A1 - Yue Liu
A1 - Ye-ting Hu
A1 - Li-feng Hu
A1 - Hai-yan Chen
A1 - Shu Zheng
A1 - Su-zhan Zhang
A1 - Ke-feng Ding
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 8
SP - 701
EP - 712
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300306
Abstract: Objective: Cancer stromal fibroblasts are important members of the cancer microenvironment. In this study, we determined the effect of sunitinib, a small molecule tyrosine kinase inhibitor, on the primary human colonic fibroblasts. Methods: Cell cycle analysis and cell proliferation assays were performed to evaluate the inhibitory effect of sunitinib in vitro. Western-blot analysis was performed to evaluate variations in the levels of phosphorylated platelet-derived growth factor receptor β (PDGFR-β), Akt, and ERK proteins. Co-injection of SW620 cells and colonic fibroblasts in nude mice was employed to test anti-growth efficacy in vivo. Results: Sunitinib was found to effectively inhibit the growth of primary colonic fibroblasts. Low-dose sunitinib blocked the PDGF-BB-induced cell proliferation and PDGFR-β signaling. Co-injection of SW620 cells and colonic fibroblasts in nude mice generated greater tumor volumes than single injection of SW620 cells. Sunitinib treatment inhibited the SW620 cell+colonic fibroblast tumor growth more effectively than treatment of 5-fluorouracil. Conclusions: sunitinib mesylate inhibited the proliferation of primary human colonic fibroblasts through target-inhibited PDGFR signaling in vitro and in vivo.
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CLC number: R735.3+4
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-06-13
Cited: 3
Clicked: 14328
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