Similar articles

Abstract: The effects of two different external carbon sources (acetate and ethanol) and electron acceptors (dissolved oxygen, nitrate, and nitrite) were investigated under aerobic and anoxic conditions with non-acclimated process biomass from a full-scale biological nutrient removal-activated sludge system. When acetate was added as an external carbon source, phosphate release was observed even in the presence of electron acceptors. The release rates were 1.7, 7.8, and 3.5 mg P/(g MLVSS·h) (MLVSS: mixed liquor volatile suspended solids), respectively, for dissolved oxygen, nitrate, and nitrite. In the case of ethanol, no phosphate release was observed in the presence of electron acceptors. Results of the experiments with nitrite showed that approximately 25 mg NO₂-N/L of nitrite inhibited anoxic phosphorus uptake regardless of the concentration of the tested external carbon sources. Furthermore, higher denitrification rates were obtained with acetate (1.4 and 0.8 mg N/(g MLVSS·h)) compared to ethanol (1.1 and 0.7 mg N/ (g MLVSS·h)) for both anoxic electron acceptors (nitrate and nitrite).

不同外加碳源和电子受体对生物营养盐去除工艺中反硝化和除磷过程的影响

[1]Adouani N, Lendormi T, Limousy L, et al., 2010. Effect of the carbon source on N₂O emissions during biological denitrification. Resour Conserv Recy, 54(5):299-302.

[2]Ahn J, Daidou T, Tsuneda S, et al., 2001. Metabolic behavior of denitrifying phosphate-accumulating organisms under nitrate and nitrite electron acceptor conditions. J Biosci Bioeng, 92(5):442-446.

[3]Ahn J, Daidou T, Tsuneda S, et al., 2002. Transformation of phosphorus and relevant intracellular compounds by a phosphorus accumulating enrichment culture in the presence of both the electron acceptor and electron donor. Biotechnol Bioeng, 79(1):83-93.

[4]Anthonisen AC, Loehr RC, Prakasam TBS, et al., 1976. Inhibition of nitrification by ammonia and nitrous acid. J Water Pollut Contr Fed, 48(5):835-852.

[5]APHA (American Public Health Association), 1998. Standard Methods for the Examination of Water and Wastewater, 20th Ed. American Public Health Association, Washington, DC, USA.

[6]Constantin H, Fick M, 1997. Influence of C-sources on the denitrification rate of a high-nitrate concentrated industrial wastewater. Water Res, 31(3):583-589.

[7]Council of the European Union, 1991. Council Directive 91/271/EEC of 21 May 1991 concerning urban wastewater treatment. Off J Eur Union, 135:40-52.

[8]Guerrero J, Guisasola A, Baeza JA, 2011. The nature of the carbon source rules the competition between PAO and denitrifiers in systems for simultaneous biological nitrogen and phosphorus removal. Water Res, 45(16):4793-4802.

[9]Guerrero J, Taya C, Guisasola A, et al., 2012. Understanding the detrimental effect of nitrate presence on EBPR systems: effect of the plant configuration. J Chem Technol Biotechnol, 87(10):1508-1511.

[10]Guisasola A, Pijuan M, Baeza JA, et al., 2004. Aerobic phosphorus release linked to acetate uptake in bio-P sludge: process modelling using oxygen uptake rate. Biotechnol Bioeng, 85(7):722-733.

[11]Hagman M, Nielsen JL, Nielsen PH, et al., 2008. Mixed carbon sources for nitrate reduction in activated sludge-identification of bacteria and process activity studies. Water Res, 42(6-7):1539-1546.

[12]Hu JY, Ong SL, Ng WJ, et al., 2003. A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptor. Water Res, 37(14):3463-3471.

[13]Isaacs SH, Henze M, 1995. Controlled carbon source addition to an alternating nitrification-denitrification wastewater treatment process including biological P removal. Water Res, 29(1):77-89.

[14]Kampas P, Parsons SA, Pearce P, et al., 2009. An internal carbon source for improving biological nutrient removal. Bioresour Technol, 100(1):149-154.

[15]Kristensen GH, Jørgensen PE, Henze M, 1992. Characterization of functional microorganism groups and substrate in activated sludge and wastewater by AUR, NUR and OUR. Water Sci Technol, 25(6):43-57.

[16]Kujawa K, Klapwijk B, 1999. A method to estimate denitrification potential for predenitrification systems using NUR batch tests. Water Res, 33(10):2291-2300.

[17]Li QH, Li P, Zhu PP, et al., 2008. Effects of exogenous organic carbon substrates on nitrous oxide emissions during the denitrification process of sequence batch reactors. Environ Eng Sci, 25(8):1221-1228.

[18]Meinhold J, Arnold E, Isaacs S, 1999. Effect of nitrite on anoxic phosphorus uptake in biological phosphorus removal activated sludge. Water Res, 33(8):1871-1883.

[19]Metcalf & Eddy Inc., Tchobanoglous G, Burton FL, et al., 2003. Wastewater Engineering: Treatment and Reuse, 4th Ed. McGraw-Hill Higher Education, New York.

[20]Morgan-Sagastume F, Nielsen JL, Nielsen PH, 2008. Substrate-dependent denitrification of abundant probe-defined denitrifying bacteria in activated sludge. FEMS Microbiol Ecol, 66(2):447-461.

[21]Peng YZ, Ma Y, Wang SY, 2007. Denitrification potential enhancement by addition of external carbon sources in a pre-denitrification process. J Environ Sci, 19(3):284-289.

[22]Pijuan M, Guisasola A, Baeza JA, et al., 2005. Aerobic phosphorus release linked to acetate uptake: influence of PAO intracellular storage compounds. Biochem Eng J, 26(2-3):184-190.

[23]Puig S, Coma M, van Loosdrecht MCM, et al., 2007. Biological nutrient removal in a sequencing batch reactor using ethanol as carbon source. J Chem Technol Biotechnol, 82(10):898-904.

[24]Puig S, Coma M, Monclusa H, et al., 2008. Selection between alcohols and volatile fatty acids as external carbon sources for EBPR. Water Res, 42(3):557-566.

[25]Rodríguez L, Villasenor J, Fernandez FJ, 2007. Use of agro-food wastewaters for the optimisation of the denitrification process. Water Sci Technol, 55(10):63-70.

[26]Saito T, Brdjanovic D, van Loosdrecht MCM, 2004. Effect of nitrite on phosphate uptake by phosphate accumulating organisms. Water Res, 38(17):3760-3768.

[27]Sin G, Niville K, Bachis G, et al., 2008. Nitrite effect on the phosphorus uptake activity of phosphate accumulating organisms (PAOs) in pilot-scale SBR and MBR reactors. Water SA, 34:249-260.

[28]Smolders GJF, van der Meij J, van Loosdrecht MCM, et al., 1994. Model of the anaerobic metabolism of the biological phosphorus removal process; stoichiometry and pH influence. Biotechnol Bioeng, 43(6):461-470.

[29]Swinarski M, Makinia J, Czerwionka K, et al., 2009. Comparison of the effects of conventional and alternative external carbon sources for enhancing the denitrification process. Water Environ Res, 81(9):896-906.

[30]Swinarski M, Makinia J, Stensel HD, et al., 2012. Modeling external carbon addition in biological nutrient removal processes with an extension of the International Water Association Activated Sludge Model. Water Environ Res, 84(8):646-655.

[31]Wang DB, Zheng W, Li XM, et al., 2013. Evaluation of the feasibility of alcohols serving as external carbon sources for biological phosphorus removal induced by the oxic/ extended-idle regime. Biotechnol Bioeng, 110(3):827-837.

[32]Yuan Q, Oleszkiewicz J, 2010. Interaction between denitrification and phosphorus removal in a sequencing batch reactor phosphorus removal system. Water Environ Res, 82(6):536-540.

[33]Zhou Y, Pijuan M, Yuan Z, 2007. Free nitrous acid inhibition on anoxic phosphorus uptake and denitrification by poly-phosphate accumulating organisms. Biotechnol Bioeng, 98(4):903-912.

[34]Zhou Y, Pijuan M, Yuan Z, 2008. Development of a 2-sludge, 3-stage system for nitrogen and phosphorous removal from nutrient-rich wastewater using granular sludge and biofilms. Water Res, 42(12):3207-3217.

[35]Zhou Y, Oehme A, Lim M, et al., 2011. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants. Water Res, 45(15):4672-4682.