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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-05-16
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Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
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Shuang LI, Zhenzhen CHEN, Chuanxin CHEN, Yuyu NIU. Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines[J]. Journal of Zhejiang University Science B, 2023, 24(5): 418-429.
@article{title="Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines",
author="Shuang LI, Zhenzhen CHEN, Chuanxin CHEN, Yuyu NIU",
journal="Journal of Zhejiang University Science B",
volume="24",
number="5",
pages="418-429",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200440"
}
%0 Journal Article
%T Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
%A Shuang LI
%A Zhenzhen CHEN
%A Chuanxin CHEN
%A Yuyu NIU
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 5
%P 418-429
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200440
TY - JOUR
T1 - Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
A1 - Shuang LI
A1 - Zhenzhen CHEN
A1 - Chuanxin CHEN
A1 - Yuyu NIU
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 5
SP - 418
EP - 429
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200440
Abstract: Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to monkey cells and provides guidance for exploring chemical cocktails for monkey stem cell line derivation.
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