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Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone
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Wei HU1*, Tianyi FEI2*, Zhichao LIU2, Yude DING1, Mengfei YU2, Fan YANG1, Feiya ZHAO1. Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone",
author="Wei HU1*, Tianyi FEI2*, Zhichao LIU2, Yude DING1, Mengfei YU2, Fan YANG1, Feiya ZHAO1",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500254"
}
%0 Journal Article
%T Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone
%A Wei HU1*
%A Tianyi FEI2*
%A Zhichao LIU2
%A Yude DING1
%A Mengfei YU2
%A Fan YANG1
%A Feiya ZHAO1
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2500254
TY - JOUR
T1 - Single-cell RNA sequencing guided reactive oxygen species scavenging hydrogel design for regeneration of osteoporotic bone
A1 - Wei HU1*
A1 - Tianyi FEI2*
A1 - Zhichao LIU2
A1 - Yude DING1
A1 - Mengfei YU2
A1 - Fan YANG1
A1 - Feiya ZHAO1
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2500254
Abstract: The pathological microenvironment of osteoporosis poses a substantial clinical challenge for bone defect regeneration. Through single-cell RNA sequencing (scRNA-seq) analysis, we identified a reactive oxygen species (ROS)-overloading osteoblast subpopulation as a critical pathological feature of osteoporotic niches. Guided by scRNA-seq analysis, we engineered a microenvironment-adaptive hydrogel system through precise integration of antioxidant curcumin-encapsulated zeolitic imidazolate framework-8 nanoparticles (CCM@ZIF-8 NPs) within photo-crosslinkable alginate methacrylate (AlgMA) hydrogel (AlgMA/CCM@ZIF-8). This engineered design exhibited dual functions: effectively scavenging ROS in bone marrow-derived mesenchymal stem cells (BMSCs) while simultaneously suppressing osteoclast differentiation. The osteo-regenerative superiority of the AlgMA/CCM@ZIF-8 nanocomposite hydrogel was conclusively demonstrated in bone defect models of osteoporotic mice. This scRNA-seq-informed engineering strategy offers a promising approach for developing pathophysiology-adapted biomaterials to promote regenerative repair in osteoporotic bone defects.
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