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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.567-569

http://doi.org/10.1631/jzus.2002.0567


Pepsin nanofilm self-assembly on the positively charged poly (ethyoene terephthalate) substrate


Author(s):  XU Li-heng, CHEN Zhi-chun, LI Yang-mei, LU De-shui, LIN Xian-fu, YUAN Jun

Affiliation(s):  The Center of Analysis and Measurement, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   xlheng@hotmail.com

Key Words:  Pepsin, Self-assembly, PET-NH3+ substrate, XPS and AFM


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XU Li-heng, CHEN Zhi-chun, LI Yang-mei, LU De-shui, LIN Xian-fu, YUAN Jun. Pepsin nanofilm self-assembly on the positively charged poly (ethyoene terephthalate) substrate[J]. Journal of Zhejiang University Science A, 2002, 3(5): 567-569.

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author="XU Li-heng, CHEN Zhi-chun, LI Yang-mei, LU De-shui, LIN Xian-fu, YUAN Jun",
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%A LIN Xian-fu
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A1 - LU De-shui
A1 - LIN Xian-fu
A1 - YUAN Jun
J0 - Journal of Zhejiang University Science A
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DOI - 10.1631/jzus.2002.0567


Abstract: 
pepsin was assembled on the surface of prepared poly (ethylene terephthalate) (PET-NH3+) substrates. The composition and structure of the pepsin / PET-NH3+ assembling films in different condition were characterized by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM).

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Chen, W., McCarthy, T. J., 1997. Layer by layer deposition: A tool for polymer surface modification. Macromolecules, 30:78-86

[2] Decher, G., Hong, J., Schmitt, J., 1992. Buildup of ultrathin multilayer films by a self-assembly process:

[3] He, L., Lin, X.F., Lu, D.S., 2001. Topographies of organized monolayer of α-amylase observed by atomic force microscopy. Chinese Chemical Letter, 12: 87-90

[4] Li, W., Wang, Z., Sun, C., 2000. Fabrication of multilayer films containing horseradish peroxidase and polycation-bearing os complex by means of electrostatic layer-by-layer adsorption and its application as a hydrogen peroxide sensor. Analytica Chimical Acta, 418:225-232

[5] Lin, X.F., He, L., Xu, L.H., 2000. The lipase monolayer film self-assembly on the negatively charged poly (ethylene terephthalate) Substrate. Chinese Chemical Letters,11: 267-270

[6] Lvov, Y., Möihwald, H.,1999. Protein Architecture: Interfacing Molecular Assemblies and Immobilization Biotechnology. MARCEL DEKKER, INC, p.169-191

[7] Stellmach, B., (translated by J.Y.Qian), 1992. Bestimmungsmethoden Enzyme. Chinese Light Industry Press, p.210

[8] Sun, Y.P., Zhang, X., Shen, J.C., 1996. Fabrication of ultrothin film containing bienzyme of glucose oxidase and glucoamylase based on electrostatic interaction and its potential application as a matose sensor. Macromol. Chem. Phys., 197:147

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