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Received: 2004-07-14

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.11-13


Construction of a eukaryotic expression plasmid of Humanin

Author(s):  LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi

Affiliation(s):  Department of Neurology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   lobenyan@263.net

Key Words:  Humanin, Alzheimer's disease, Eukaryotic expression

LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi. Construction of a eukaryotic expression plasmid of Humanin[J]. Journal of Zhejiang University Science B, 2005, 6(1): 11-13.

@article{title="Construction of a eukaryotic expression plasmid of Humanin",
author="LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Construction of a eukaryotic expression plasmid of Humanin
%A LUO Ben-yan
%A CHEN Xiang-ming
%A CHEN Feng
%J Journal of Zhejiang University SCIENCE B
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%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0011

T1 - Construction of a eukaryotic expression plasmid of Humanin
A1 - LUO Ben-yan
A1 - CHEN Xiang-ming
A1 - TANG Min
A1 - CHEN Feng
A1 - CHEN Zhi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 11
EP - 13
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0011

Objective: To construct a eukaryotic expression plasmid pcDNA3.1(-)-humanin. Methods: The recombinant plasmid pGEMEX-1-humanin was digested with restriction endonucleases BamH I and Hind III and the humanin gene fragments, about 100 bp length, were obtained. Then the humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-humanin were identified by sequencing. Results: Recombinant plasmid DNA successfully produced a band which had the same size as that of the humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. Conclusions: A eukaryotic expression plasmid of humanin was successfully constructed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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