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Received: 2004-04-29

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.2 P.132-136


Representative appressorium stage cDNA library of Magnaporthe grisea

Author(s):  LU Jian-ping, LIU Tong-bao, YU Xiao-yun, LIN Fu-cheng

Affiliation(s):  School of Life Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   fuchenglin@zju.edu.cn

Key Words:  Magnaporthe grisea, Appressorium, cDNA library

LU Jian-ping, LIU Tong-bao, YU Xiao-yun, LIN Fu-cheng. Representative appressorium stage cDNA library of Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2005, 6(2): 132-136.

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author="LU Jian-ping, LIU Tong-bao, YU Xiao-yun, LIN Fu-cheng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Representative appressorium stage cDNA library of Magnaporthe grisea
%A LU Jian-ping
%A LIU Tong-bao
%A YU Xiao-yun
%A LIN Fu-cheng
%J Journal of Zhejiang University SCIENCE B
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%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0132

T1 - Representative appressorium stage cDNA library of Magnaporthe grisea
A1 - LU Jian-ping
A1 - LIU Tong-bao
A1 - YU Xiao-yun
A1 - LIN Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 132
EP - 136
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0132

A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMARTTM cDNA library containing 2.37×106 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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