Full Text:   <3358>

CLC number: Q78

On-line Access: 

Received: 2009-01-11

Revision Accepted: 2009-03-19

Crosschecked: 2009-05-06

Cited: 22

Clicked: 6174

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.6 P.434-444


MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae

Author(s):  Tong-bao LIU, Guo-qing CHEN, Hang MIN, Fu-cheng LIN

Affiliation(s):  State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   fuchenglin@zju.edu.cn

Key Words:  Magnaporthe oryzae, Fasciclin, MoFLP1, Cellular localization, Conidiation, Pathogenicity

Tong-bao LIU, Guo-qing CHEN, Hang MIN, Fu-cheng LIN. MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae[J]. Journal of Zhejiang University Science B, 2009, 10(6): 434-444.

@article{title="MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae",
author="Tong-bao LIU, Guo-qing CHEN, Hang MIN, Fu-cheng LIN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae
%A Tong-bao LIU
%A Guo-qing CHEN
%A Hang MIN
%A Fu-cheng LIN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 6
%P 434-444
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920017

T1 - MoFLP1, encoding a novel fungal fasciclin-like protein, is involved in conidiation and pathogenicity in Magnaporthe oryzae
A1 - Tong-bao LIU
A1 - Guo-qing CHEN
A1 - Hang MIN
A1 - Fu-cheng LIN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 6
SP - 434
EP - 444
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920017

fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity in M. oryzae.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Adachi, H., Tsujimoto, M., 2002. FEEL-1, a novel scavenger receptor with in vitro bacteria-binding and angiogenesis-modulating activities. J. Biol. Chem., 277(37):34264-34270.

[2] Adachi, K., Hamer, J.E., 1998. Divergent camp signaling pathways regulate growth and pathogenesis in the rice blast fungus, Magnaporthe grisea. Plant Cell, 10(8): 1361-1373.

[3] Ahn, N., Kim, S., Choi, W., Im, K.H., Lee, Y.H., 2004. Extracellular matrix protein gene, EMP1, is required for appressorium formation and pathogenicity of the rice blast fungus, Magnaporthe grisea. Mol. Cells, 17(1): 166-173.

[4] Bairoch, A., Bucher, P., Hofmann, K., 1997. The PROSITE database, its status in 1997. Nucleic Acids Res., 25(1): 217-221.

[5] Bastiani, M.J., Harrelson, A.L., Snow, P.M., Goodman, C.S., 1987. Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper. Cell, 48(5):745-755.

[6] Bonman, J.M., Vergel, D.D.T., Khin, M.M., 1986. Physiologic specialization of Pyricularia oryzae in the Philippines. Plant Dis., 70(8):767-769.

[7] Carr, M.D., Bloemink, M.J., Dentten, E., Whelan, A.O., Gordon, S.V., Kelly, G., Frenkiel, T.A., Hewinson, R.G., Williamson, R.A., 2003. Solution structure of the Mycobacterium tuberculosis complex protein MPB70: from tuberculosis pathogenesis to inherited human corneal desease. J. Biol. Chem., 278(44):43736-43743.

[8] Carroll, A.M., Sweigard, J.A., Valent, B., 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genet. Newslett., 41:22.

[9] Dahiya, P., Findlay, K., Roberts, K., McCann, M.C., 2006. A fasciclin-domain containing gene, ZeFLA11, is expressed exclusively in xylem elements that have reticulate wall thickenings in the stem vascular system of Zinnia elegans cv Envy. Planta, 223(6):1281-1291.

[10] de Jong, J.C., McCormack, B.J., Smirnoff, N., Talbot, N.J., 1997. Glycerol generates turgor in rice blast. Nature, 389(6648):244-245.

[11] Dyrløv Bendtsen, J.D., Nielsen, H., von Heijne, G., Brunak, S., 2004. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol., 340(4):783-795.

[12] Ebbole, D.J., 2007. Magnaporthe as a model for understanding host-pathogen interactions. Annu. Rev. Phytopathol., 45(1):437-456.

[13] Eisenhaber, B., Bork, P., Eisenhaber, F., 1998. Sequence properties of GPI-anchored proteins near the omega-site: constraints for the polypeptide binding site of the putative transamidase. Protein Engineering Design and Selection, 11(12):1155-1161.

[14] Elkins, T., Hortsch, M., Bieber, A.J., Snow, P.M., Goodman, C.S., 1990. Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting. J. Cell Biol., 110(5):1825-1832.

[15] Faik, A., Abouzouhair, J., Sarhan, F., 2006. Putative fasciclin-like arabinogalactan-proteins (FLA) in wheat (Triticum aestivum) and rice (Oryza sativa): identification and bioinformatic analyses. Mol. Gen. Genomics, 276(5): 478-494.

[16] Hashimoto, K., Noshiro, M., Ohno, S., Kawamoto, T., Satakeda, H., Akagawa, Y., Nakashima, K., Okimura, A., Ishida, H., Okamoto, T., et al., 1997. Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen. Biochim. Biophys. Acta, 1355(3): 303-314.

[17] Huber, O., Sumper, M., 1994. Algal-CAMs: isoforms of a cell adhesion molecule in embryos of the alga volvox with homology to Drosophila fasciclin I. EMBO J., 13(18): 4212-4222.

[18] Johnson, K.L., Jones, B.J., Bacic, A., Schultz, C.J., 2003. The fasciclin-like arabinogalactan proteins of Arabidopsis. A multigene family of putative cell adhesion molecules. Plant Physiol., 133(4):1911-1925.

[19] Kawamoto, T., Noshiro, M., Shen, M., Nakamasu, K., Hashimoto, K., Kawashima-Ohya, Y., Gotoh, O., Kato, Y., 1998. Structural and phylogenetic analyses of RGD-CAPrbig-h3, a fasciclin-like adhesion protein expressed in chick chondrocytes. Biochimica et Biophysica Acta, 1395:288-292.

[20] Kikuma, T., Ohneda, M., Arioka, M., Kitamoto, K., 2006. Functional analysis of the ATG8 homologue Aoatg8 and role of autophagy in differentiation and germination in Aspergillus oryzae. Eukaryot. Cell, 5(8):1328-1336.

[21] Kim, J.E., Kim, S.J., Lee, B.H., Park, R.W., Kim, K.S., Kim, I.S., 2000. Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-beta-induced gene, beta-ig-h3. J. Biol. Chem., 275(40):30907-30915.

[22] Kim, S., Ahn, I.P., Rho, H.S., Lee, Y.H., 2005. MHP1, a Magnaporthe grisea hydrophobin gene, is required for fungal development and plant colonization. Mol. Microbiol., 57(5):1224-1237.

[23] Lee, K., Singh, P., Chung, W.C., Ash, J., Kim, T.S., Hang, L., Park, S., 2006. Light regulation of asexual development in the rice blast fungus, Magnaporthe oryzae. Fungal Genet. Biol., 43(10):694-706.

[24] Li, L., Xue, C., Bruno, K., Nishimura, M., Xu, J.R., 2004. Two PAK kinase genes, CHM1 and MST20, have distinct functions in Magnaporthe grisea. Mol. Plant Microbe Interact., 17(5):547-556.

[25] Liu, D., Tu, L., Li, Y., Wang, L., Zhu, L., Zhang, X., 2008. Genes encoding fasciclin-like arabinogalactan proteins are specifically expressed during cotton fiber development. Plant Mol. Biol. Rep., 26(2):98-113.

[26] Liu, T.B., Lu, J.P., Min, H., Lin, F.C., 2008. A simple and effective method for total RNA isolation of appressoria in Magnaporthe oryzae. J. Zhejiang Univ. Sci. B, 9(10): 811-817.

[27] Liu, X.H., Lu, J.P., Zhang, L., Dong, B., Min, H., Lin, F.C., 2007. Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis. Eukaryot. Cell, 6(6):997-1005.

[28] Lu, J.P., Liu, T.B., Lin, F.C., 2005a. Identification of mature appressorium-enriched transcripts in Magnaporthe grisea, the rice blast fungus, using suppression subtractive hybridization. FEMS Microbiol. Lett., 245(1):131-137.

[29] Lu, J.P., Liu, T.B., Liu, X.Y., Lin, F.C., 2005b. Representative appressorium stage cDNA library of Magnaporthe grisea. J. Zhejiang Univ. Sci. B, 6(2):132-136.

[30] Lu, J.P., Duan, Z.B., Liu, T.B., Lin, F.C., 2007a. Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea. J. Zhejiang Univ. Sci. B, 8(9):661-665.

[31] Lu, J.P., Feng, X.X., Liu, X.H., Lu, Q., Wang, H.K., Lin, F.C., 2007b. Mnh6, a nonhistone protein, is required for fungal development and pathogenicity of Magnaporthe grisea. Fungal Genet. Biol., 44(9):819-829.

[32] Lu, Q., Lu, J.P., Li, X.D., Liu, X.H., Min, H., Lin, F.C., 2008. Magnaporthe oryzae MTP1 gene encodes a type III transmembrane protein involved in conidiation and conidial germination. J. Zhejiang Univ. Sci. B, 9(7): 511-519.

[33] Miyazaki, Y., Kaneko, S., Sunagawa, M., Shishido, K., Yamazaki, T., Nakamura, M., Babasaki, K., 2007. The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes. Curr. Genet., 51(6):367-375.

[34] Nishimura, M., Park, G., Xu, J.R., 2003. The G-beta subunit MGB1 is involved in regulating multiple steps of infection-related morphogenesis in Magnaporthe grisea. Mol. Microbiol., 50(1):231-243.

[35] Ohno, S., Doi, T., Tsutsumi, S., Okada, Y., Yoneno, K., Kato, Y., Tanne, K., 2002. RGD-CAP (βig-h3) is expressed in precartilage condensation and in prehypertrophic chondrocytes during cartilage development. Biochim. Biophys. Acta, 1572(1):114-122.

[36] Ou, S.H., 1985. Rice Diseases, 2nd Ed. Commonwealth Mycological Institute, Kew, UK.

[37] Park, S.W., Bae, J.S., Kim, K.S., Park, S.H., Lee, B.H., Choi, J.Y., Park, J.Y., Ha, S.W., Kim, Y.L., Kwon, T.H., Kim, I.S., Park, R.W., 2004. Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with α3β1 integrin. Exp. Mol. Med., 36(3):211-219.

[38] Paulsrud, P., Lindblad, P., 2002. Fasciclin domain proteins are present in nostoc symbionts of lichens. Appl. Environ. Microbiol., 68(4):2036-2039.

[39] Politz, O., Gratchev, A., McCourt, P.A.G., Schledzewski, K., Guillot, P., Johansson, S., Svineng, G., Franke, P., Kannicht, C., Kzhyshkowaka, J., et al., 2002. Stabilin-1 and -2 constitute a novel family of fasciclin-like hyaluronan receptor homologues. Biochem. J., 362(1): 155-164.

[40] Sambrook, J., Fritsch, E.F., Maniatis, T., 2002. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, New York, N.Y.

[41] Sato, K., Nishi, N., Nomizu, M., 2004. Characterization of a fasciclin I-like protein with cell attachment activity from sea urchin (Strongylocentrotus intermedius) ovaries. Arch. Biochem. Biophys., 424(1):1-10.

[42] Schultz, C.J., Johnson, K.L., Currie, G., Bacic, A., 2000. The classical arabinogalactan protein gene family of Arabidopsis. Plant Cell, 12(9):1751-1767.

[43] Skonier, J., Neubauer, M., Madisen, L., Bennett, K., Plowman, G.D., Purchio, A.F., 1992. cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta. DNA Cell Biol., 11(7): 511-522.

[44] Takeshita, S., Kikuno, R., Tezuka, K., Amann, E., 1993. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I. Biochem. J., 294(1):271-278.

[45] Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell, 5(11):1575-1590.

[46] Thompson, J.D., Higgins, D.G., Gibson, T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22(22):4673-4680.

[47] Valent, B., 1990. Rice blast as a model system for plant pathology. Phytopathology, 80(1):33-36.

[48] Veneault-Fourrey, C., Barooah, M.K., Egan, M.J., Talbot, N.J., 2006. Autophagic fungal cell death is necessary for infection by the rice blast fungus. Science, 312(5773): 580-583.

[49] Yi, M., Park, J.H., Ahn, J.H., Lee, Y.H., 2008. MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus Magnaporthe oryzae. Fungal Genet. Biol., 45(8): 1172-1181.

[50] Zhao, X., Mehrabi, R., Xu, J.R., 2007. Mitogen-activated protein kinase pathways and fungal pathogenesis. Eukaryot. Cell, 6(10):1701-1714.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE