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Received: 2006-01-09

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.445-451


Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients

Author(s):  XU Dong, LI Xu-fen, ZHENG Shu, JIANG Wen-zhi

Affiliation(s):  Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   honokiol@gmail.com, zhengshu@zju.edu.cn

Key Words:  Colorectal carcinoma, Real-time RT-PCR, CEA mRNA, CK20 mRNA, CK19 mRNA

XU Dong, LI Xu-fen, ZHENG Shu, JIANG Wen-zhi. Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients[J]. Journal of Zhejiang University Science B, 2006, 7(6): 445-451.

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author="XU Dong, LI Xu-fen, ZHENG Shu, JIANG Wen-zhi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients
%A XU Dong
%A LI Xu-fen
%A JIANG Wen-zhi
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 6
%P 445-451
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0445

T1 - Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients
A1 - XU Dong
A1 - LI Xu-fen
A1 - ZHENG Shu
A1 - JIANG Wen-zhi
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 6
SP - 445
EP - 451
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0445

This study is aimed at establishing a sensitive approach to detect disseminated tumor cells in peripheral blood and evaluate its clinical significance. A total of 198 blood samples including 168 from colorectal carcinoma (CRC) patients and 30 from healthy volunteers were examined by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to evaluate the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and cytokeratin 19 (CK19) mRNA. CEA mRNA was detected in 35.8% of patients and 3.3% of controls, CK20 mRNA in 28.3% of patients and 6.7% of controls, and CK19 mRNA in 41.9% of patients and 3.3% of controls. CEA and CK20 mRNA positive ratio increased with the advancing Dukes stages, but there was no significant difference in positive ratio between any two stages (P>0.05). Also, relatively high positive ratio of CEA, CK20 and CK19 mRNA expression was observed in some CRC patients with earlier Dukes stages. A higher positive ratio was obtained when two or three detection markers were combined compared to a single marker. Our study indicates that quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood is a valuable tool for monitoring early stage dissemination of CRC cells in blood circulation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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