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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.7 P.596-602


Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy

Author(s):  SONG Zeng-fu, WU Tian-xing, CAI Li-sheng, ZHANG Li-jing, ZHENG Xiao-dong

Affiliation(s):  School of Animal Sciences, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   sdauszf@sohu.com, wutx@tianbang.com

Key Words:  Clostridium butyricum, Growth performance, Humoral immune response, Miichthys miiuy

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SONG Zeng-fu, WU Tian-xing, CAI Li-sheng, ZHANG Li-jing, ZHENG Xiao-dong. Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy[J]. Journal of Zhejiang University Science B, 2006, 7(7): 596-602.

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author="SONG Zeng-fu, WU Tian-xing, CAI Li-sheng, ZHANG Li-jing, ZHENG Xiao-dong",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy
%A SONG Zeng-fu
%A WU Tian-xing
%A CAI Li-sheng
%A ZHANG Li-jing
%A ZHENG Xiao-dong
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 7
%P 596-602
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0596

T1 - Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy
A1 - SONG Zeng-fu
A1 - WU Tian-xing
A1 - CAI Li-sheng
A1 - ZHANG Li-jing
A1 - ZHENG Xiao-dong
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 7
SP - 596
EP - 602
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0596

The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200~260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets: basal diet only (control) or supplemented of the basal diet with C. butyricum at doses of 103 (CB1), 105 (CB2), 107 (CB3) or 109 (CB4) CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation (P<0.05), acid phosphatases activity was increased significantly (P<0.05) at the doses of 109 CFU/g. Serum lysozyme activity peaked at dose of 107 CFU/g and in the skin mucus at dose of 109 CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 103 CFU/g (P<0.05). The growth at the dose of 109 CFU/g was higher than that of the control (P<0.05). It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Asada, N., Fukumitsu, T., Fujimoto, K., Masuda, K.L., 1993. Activation of prophenoloxidase with 2-propanol and other organic compounds in Drosophila melanogaster. Insect Biochem. Mol. Biol., 23(4):515-520.

[2] Ashida, M., Säderhäll, K., 1984. The prophenoloxidase activating system in crayfish. Comp. Biochem. Physiol., 77B:21-26.

[3] Asokan, R., Arumugam, M., Mullainadhan, P., 1997. Activation of prophenoloxidase in the plasma and haemocytes of the marine mussel Perna viridis Linnaeus. Dev. Comp. Immunol., 21(1):1-12.

[4] Bowden, T.J., Adamson, K., MacLachlan, P., Pert, C.C., Bricknell, I.R., 2003. Long-term study of antibody response and injection-site effects of oil adjuvants in Atlantic halibut (Hippoglossus hippoglossus L.). Fish & Shellfish Immunology, 14(4):363-369.

[5] Chen, S.C., Yoshida, T., Adams, A., Thompson, K.D., Richards, R.H., 1996. Immune response of rainbow trout to extracellular products of Mycobacterium sp. J. Aquat. Anim. Health, 8(3):216-222.

[6] Chipman, D.M., Sharon, N., 1969. Mechanism of lysozyme action. Science, 165(892):454-465.

[7] Cuesta, A., Meseguer, J., Esteban, M.A., 2004. Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.). Veterinary Immunology and Immunopathology, 101(3-4):203-210.

[8] Ellis, A.E., 1990. Lysozyme Assays. In: Stolen, J.S., Fletcher, D.P., Anderson, B.S., Roberson, B.S. (Eds.), Techniques in Fish Immunology. Fair Haven, SOS Publications, USA, p.101-103.

[9] Fuller, R., 1989. Probiotics in man and animals. J. Appl. Bacteriol., 66(5):365-378.

[10] Gatesoupe, F.J., 1999. The use of probiotics in aquaculture. Aquaculture, 180(1-2):147-165.

[11] Grinde, B., 1989. Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens. J. Fish Dis., 12(2):95-104.

[12] Grinde, B., Lie, Ø., Poppe, T., Salte, R., 1988. Species and individual variation in lysozyme activity in fish of interest in aquaculture. Aquaculture, 68(4):299-304.

[13] Han, L.K., Lee, S.C., Lee, J.H., Lee, K.K., Lee, J.C., 1984. Studies on the growth promoting effects of probiotics II. The effect of Clostridium butyricum ID on the performance and changes in the microbial flora of the faeces and intestinal of the broiler chickens. Korean Journal of Animal Science, 26(2):158-165.

[14] Ito, I., Hayashi, T., Iguchi, A., Endo, H., Nakao, M., Kato, S., Nabeshima, T., Ogura, Y., 1997. Effects of administration of Clostridium butyricum to patients receiving long-term tube feeding. Jpn. J. Geriat., 34(4):298-304.

[15] Kamiya, S., Taguchi, H., Yamaguchi, H., Osaki, T., Takahashi, M., Nakamura, S., 1997. Bacterioprophylaxis using Clostridium butyricum for lethal caecitis by Clostridium difficile. Rev. Med. Miclobiol., 8:S57-S59.

[16] Lara-Flores, M., Olvera-Novoa, M.A., Guzmán-Méndez, B.E., Lόpez-Madrid, W., 2003. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture, 216(1-4):193-201.

[17] Møyner, K., Roed, K.H., Sevatdal, S., Heum, M., 1993. Changes in non-specific immune parameters in Atlantic salmon, Salmo salar L. induced by Aeromonas salmonicida infection. Fish & Shellfish Immunol., 3(4):253-265.

[18] Panigrahi, A., Kiron, V., Kobayashi, T., Puangkaew, J., Satoh, S., Sugita, H., 2004. Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Veterinary Immunology and Immunopathology, 102(4):379-388.

[19] Perdigon, G., Alvarez, S., de Macias, M.E.N., 1990. The oral administration of lactic acid bacteria increase the mucosal intestinal immunity in response to enteropathogens. Journal of Food Protection, 53(5):404-410.

[20] Pipe, R.K., 1990. Hydrolytic enzymes associated with the granular haemocytes of the marine mussel Mytilus edulis. Histochem. J., 22(11):595-603.

[21] Ross, D.A., Wilson, M.R., Miller, N.W., Clem, L.W., Warr, G.W., 1998. Evolutionary variation of immunoglobulin µ-heavy chain RNA processing pathways: origins, effects, and implications. Immunol. Rev., 166(1):143-151.

[22] Sakai, M., Yoshida, T., Atsuta, S., Kobayashi, M., 1995. Enhancement of resistance to vibriosis in rainbow trout, Oncorhynchus mykiss Walbaum by oral administration Clostridium butyricum bacterin. J. Fish Dis., 18(2):187-190.

[23] Smith, V.J., Säderhäll, K., 1991. A comparison of phenoloxidase activity in the blood of marine invertebrates. Dev. Comp. Immunol., 15(4):251-261.

[24] Sugumaran, M., Nellaiappan, K., 1991. Lysolecithinda potent activator of prophenoloxidase from the hemolymph of the lobster, Homarus americanus. Biochem. Biophys. Res. Commun., 176(3):1371-1376.

[25] Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W., 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64(4):655-671.

[26] Wang, Y.B., Xu, Z.R., 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology, 127(3-4):283-292.

[27] Watts, M., Munday, B.L., Burke, C.M., 2001. Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau. Fish and Shellfish Immunology, 11(6):491-503.

[28] Yang, G.P., 1998. Study on the biological property of Clostridium butycirum. Chinese Journal of Microceology, 5(5):306-308.

[29] Young, C.H., Kaneda, S., Mikami, Y., Arai, T., Igarashi, K., Saito, M., Miyoshi, T., Fuse, A., 1987. Protection activity induced by the bacterial vaccine, heat-killed Closteridium butyricum against Candida albicans infections in mice. Jpn. J. Med. Mycol., 28(2):262-269.

[30] Yousif, A.N., Albright, L.J., Evelyn, T.P.T., 1994. In vitro evidence for the antibacterial role of lysozyme in salmonid eggs. Dis. Aquat. Org., 19(1):15-19.

[31] Ziaei-Nejad, S., Rezaei, M.H., Takami, G.A., Lovett, D.L., Mirvaghefi, A.R., Shakouri, M., 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252(2-4):516-524.

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