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CLC number: TU991.21

On-line Access: 2017-04-05

Received: 2016-04-15

Revision Accepted: 2017-02-09

Crosschecked: 2017-03-09

Cited: 0

Clicked: 3208

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jing-qing Liu

http://orcid.org/0000-0001-5596-0365

Li-ping Lou

http://orcid.org/0000-0002-5148-9323

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Journal of Zhejiang University SCIENCE A 2017 Vol.18 No.4 P.313-328

http://doi.org/10.1631/jzus.A1600316


Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems


Author(s):  Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou

Affiliation(s):  College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   loulp@zju.edu.cn

Key Words:  Drinking water distribution system (DWDS), Biofilms, Flushing, Metal pipe


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Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou. Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems[J]. Journal of Zhejiang University Science A, 2017, 18(4): 313-328.

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author="Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou",
journal="Journal of Zhejiang University Science A",
volume="18",
number="4",
pages="313-328",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A1600316"
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%A Jing-qing Liu
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%A Hong-xi Peng
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%A Xiao-yan Zhou
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%A Hamid Bastani
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A1 - Hong-xi Peng
A1 - Bao-lan Hu
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A1 - Xiao-yan Zhou
A1 - Shang-de Qiu
A1 - Xiao-fang He
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Abstract: 
Biofilm detachment caused by flushing can result in secondary contamination in drinking water distribution systems (DWDSs). To evaluate the impact of flushing on biofilm detachment, actual water supply pipes including ductile cast iron pipes (DCIPs), gray cast iron pipes (GCIPs), and stainless steel compound pipes (SSCPs) were used in this study. Real-time quantitative polymerase chain reaction and 454 pyrosequencing were used to quantify bacteria and analyse microbial community composition, respectively. The results showed that the pipe material greatly influences the resistance of a biofilm to flushing. biofilms attached to DCIPs were able to resist quite strong flushing, while those attached to GCIPs and SSCPs were sensitive to flushing. Both flush-resistant and flush-sensitive bacteria were present in all the biofilms, but their frequency differed among the different metal pipes. Thus, the resistance to flushing of bacteria is related not only to the nature of the bacteria, but also to the pipe material. Although flushing can remove some of the biofilm and may be a good way to clean the DWDS, the shear stress needed to remove the biofilm differs among different pipe types. The results of this study provide technical support for the management and operation of DWDS.

The paper presents some good work extending knowledge on biofilms in distribution systems and follows previous publications by the contact author researching these aspects in China (e.g. Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China (2015) & Impact of pipe materials on bacterial population diversity in drinking water biofilm (2015)). It is generally well written and includes relevant references. The sample size is sufficient for this study, although perhaps samples from locations with different water supply characteristics could help reinforce the findings.

供水系统中冲刷对金属管道内壁生物膜脱落的影响作用

目的:在市政供水管网系统中,由冲刷引起的管道内壁生物膜脱落可能造成饮用水二次污染。本文旨在通过研究冲刷前后生物膜理化特性和微生物种群结构的变化,探讨不同金属管材管道和不同冲刷流速对生物膜脱落的影响,从而为管道生物安全的风险评估提供科学依据。
创新点:不同菌属的细菌对水力冲刷的敏感程度不同;各菌属对水力变化的敏感程度既与菌属本身的性质有关,也与其附着生长的管材有关。
方法:1. 采用R2A培养基平板计数、16S rDNA检测、荧光定量聚合酶链式反应及454焦磷酸测序技术。2. 通过理化指标检测与测序相结合的方式进行分析,并以丰度图和热度图的形式呈现结果。
结论:1. 球墨铸铁管内壁生物膜的抗水力剪切能力较强,能够抵抗低剪切冲刷,在高剪切力冲刷后才会明显脱落,而灰口铸铁管和不锈钢复合管内壁生物膜的抗水力剪切能力较弱,低剪切力冲刷工况便会导致生物膜的明显脱落。2. 生物膜对冲刷的抵抗作用在其群落的属水平和纲水平上有显著变化,这与群落本身和管材特性均有关联。

关键词:供水管网系统;生物膜;冲刷;金属管道

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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