Full Text:   <3002>

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CLC number: TU991.21

On-line Access: 2017-04-05

Received: 2016-04-15

Revision Accepted: 2017-02-09

Crosschecked: 2017-03-09

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Jing-qing Liu


Li-ping Lou


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Journal of Zhejiang University SCIENCE A 2017 Vol.18 No.4 P.313-328


Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems

Author(s):  Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou

Affiliation(s):  College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   loulp@zju.edu.cn

Key Words:  Drinking water distribution system (DWDS), Biofilms, Flushing, Metal pipe

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Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou. Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems[J]. Journal of Zhejiang University Science A, 2017, 18(4): 313-328.

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author="Jing-qing Liu, Zhi-feng Luo, Ke Liu, Yi-fu Zhang, Hong-xi Peng, Bao-lan Hu, Hong-xing Ren, Xiao-yan Zhou, Shang-de Qiu, Xiao-fang He, Ping Ye, Hamid Bastani, Li-ping Lou",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

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%T Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems
%A Jing-qing Liu
%A Zhi-feng Luo
%A Ke Liu
%A Yi-fu Zhang
%A Hong-xi Peng
%A Bao-lan Hu
%A Hong-xing Ren
%A Xiao-yan Zhou
%A Shang-de Qiu
%A Xiao-fang He
%A Ping Ye
%A Hamid Bastani
%A Li-ping Lou
%J Journal of Zhejiang University SCIENCE A
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%P 313-328
%@ 1673-565X
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A1600316

T1 - Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems
A1 - Jing-qing Liu
A1 - Zhi-feng Luo
A1 - Ke Liu
A1 - Yi-fu Zhang
A1 - Hong-xi Peng
A1 - Bao-lan Hu
A1 - Hong-xing Ren
A1 - Xiao-yan Zhou
A1 - Shang-de Qiu
A1 - Xiao-fang He
A1 - Ping Ye
A1 - Hamid Bastani
A1 - Li-ping Lou
J0 - Journal of Zhejiang University Science A
VL - 18
IS - 4
SP - 313
EP - 328
%@ 1673-565X
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A1600316

Biofilm detachment caused by flushing can result in secondary contamination in drinking water distribution systems (DWDSs). To evaluate the impact of flushing on biofilm detachment, actual water supply pipes including ductile cast iron pipes (DCIPs), gray cast iron pipes (GCIPs), and stainless steel compound pipes (SSCPs) were used in this study. Real-time quantitative polymerase chain reaction and 454 pyrosequencing were used to quantify bacteria and analyse microbial community composition, respectively. The results showed that the pipe material greatly influences the resistance of a biofilm to flushing. biofilms attached to DCIPs were able to resist quite strong flushing, while those attached to GCIPs and SSCPs were sensitive to flushing. Both flush-resistant and flush-sensitive bacteria were present in all the biofilms, but their frequency differed among the different metal pipes. Thus, the resistance to flushing of bacteria is related not only to the nature of the bacteria, but also to the pipe material. Although flushing can remove some of the biofilm and may be a good way to clean the DWDS, the shear stress needed to remove the biofilm differs among different pipe types. The results of this study provide technical support for the management and operation of DWDS.

The paper presents some good work extending knowledge on biofilms in distribution systems and follows previous publications by the contact author researching these aspects in China (e.g. Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China (2015) & Impact of pipe materials on bacterial population diversity in drinking water biofilm (2015)). It is generally well written and includes relevant references. The sample size is sufficient for this study, although perhaps samples from locations with different water supply characteristics could help reinforce the findings.


方法:1. 采用R2A培养基平板计数、16S rDNA检测、荧光定量聚合酶链式反应及454焦磷酸测序技术。2. 通过理化指标检测与测序相结合的方式进行分析,并以丰度图和热度图的形式呈现结果。
结论:1. 球墨铸铁管内壁生物膜的抗水力剪切能力较强,能够抵抗低剪切冲刷,在高剪切力冲刷后才会明显脱落,而灰口铸铁管和不锈钢复合管内壁生物膜的抗水力剪切能力较弱,低剪切力冲刷工况便会导致生物膜的明显脱落。2. 生物膜对冲刷的抵抗作用在其群落的属水平和纲水平上有显著变化,这与群落本身和管材特性均有关联。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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