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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.7 P.512-521

http://doi.org/10.1631/jzus.B0820300


Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii


Author(s):  Rui CHEN, Shao-hong LU, Qun-bo TONG, Di LOU, Dong-yan SHI, Bing-bing JIA, Guo-ping HUANG, Jin-fu WANG

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wjfu@zju.edu.cn

Key Words:  DNA vaccine, Granule protein 4 (GRA4), Liposome, Toxoplasma gondii


Rui CHEN, Shao-hong LU, Qun-bo TONG, Di LOU, Dong-yan SHI, Bing-bing JIA, Guo-ping HUANG, Jin-fu WANG. Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii[J]. Journal of Zhejiang University Science B, 2009, 10(7): 512-521.

@article{title="Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii",
author="Rui CHEN, Shao-hong LU, Qun-bo TONG, Di LOU, Dong-yan SHI, Bing-bing JIA, Guo-ping HUANG, Jin-fu WANG",
journal="Journal of Zhejiang University Science B",
volume="10",
number="7",
pages="512-521",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820300"
}

%0 Journal Article
%T Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii
%A Rui CHEN
%A Shao-hong LU
%A Qun-bo TONG
%A Di LOU
%A Dong-yan SHI
%A Bing-bing JIA
%A Guo-ping HUANG
%A Jin-fu WANG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 7
%P 512-521
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820300

TY - JOUR
T1 - Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii
A1 - Rui CHEN
A1 - Shao-hong LU
A1 - Qun-bo TONG
A1 - Di LOU
A1 - Dong-yan SHI
A1 - Bing-bing JIA
A1 - Guo-ping HUANG
A1 - Jin-fu WANG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 7
SP - 512
EP - 521
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820300


Abstract: 
The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3.1-GRA4 (pGRA4), encoding for the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine (PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP). C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 103 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against infection of T. gondii.

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