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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.876-883

http://doi.org/10.1631/jzus.2006.B0876


Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters


Author(s):  WU Yan-min, YAN Jie, CHEN Li-li, SUN Wei-lian, GU Zhi-yuan

Affiliation(s):  Department of Stomatology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   chenlili0330@126.com

Key Words:  Periodontitis, Polymerase chain reaction, Epstein-Barr virus, Polymorphism, Restriction fragment length


WU Yan-min, YAN Jie, CHEN Li-li, SUN Wei-lian, GU Zhi-yuan. Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters[J]. Journal of Zhejiang University Science B, 2006, 7(11): 876-883.

@article{title="Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters",
author="WU Yan-min, YAN Jie, CHEN Li-li, SUN Wei-lian, GU Zhi-yuan",
journal="Journal of Zhejiang University Science B",
volume="7",
number="11",
pages="876-883",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0876"
}

%0 Journal Article
%T Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters
%A WU Yan-min
%A YAN Jie
%A CHEN Li-li
%A SUN Wei-lian
%A GU Zhi-yuan
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 876-883
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0876

TY - JOUR
T1 - Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters
A1 - WU Yan-min
A1 - YAN Jie
A1 - CHEN Li-li
A1 - SUN Wei-lian
A1 - GU Zhi-yuan
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 876
EP - 883
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0876


Abstract: 
Objective: To detect the infection frequencies of different genotypes of epstein-Barr virus (EBV) in subgingival samples from chronic periodontitis (CP) patients, and to discuss the correlation between infection with EBV and clinical parameters. Methods: Nested-PCR assay was used to detect EBV-1 and EBV-2 in subgingival samples from 65 CP patients, 65 gingivitis patients and 24 periodontally healthy individuals. The amplicons were further identified by restriction fragment length polymorphism analysis (RFLP) with endonucleases Afa I and Stu I. Clinical parameters mainly included bleeding on probing (BOP), probing depth (PD), attachment loss (AL) in six sites of the dentition. Results: In CP patients, gingivitis and periodontally healthy individuals, the infection frequencies were 47.7%, 24.6% and 16.7% for EBV-1, and 15.4%, 7.7% and 0% for EBV-2, respectively. In 2 out of the 65 CP patients co-infection of EBV-1 and EBV-2 was found. The positive rate of EBV-1 in chronic periodontitis patients was higher than that in gingivitis patients (P=0.01) and periodontally healthy individuals (P=0.01). But no significant difference was shown in EBV-1 frequency between gingivitis patients and healthy individuals (P>0.05) or in EBV-2 frequency among the three groups (P>0.05). In CP patients, higher mean BOP value was found in EBV-1 or EBV-2 positive patients than that in EBV negative ones (P<0.01), but with no statistical difference in the mean PD or AL value between EBV positive and negative patients (P>0.05). After initial periodontal treatment, 12 out of the 21 EBV-1 positive CP patients did not show detectable EBV-1 in subgingival samples. Conclusion: nPCR plus RFLP analysis is a sensitive, specific and stable method to detect EBV-1 and EBV-2 in subgingival samples. Subgingival infection with EBV-1 is closely associated with chronic periodontitis. Infection of EBV in subgingival samples was correlated with BOP.

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