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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.9 P.661-672

http://doi.org/10.1631/jzus.B1000155


Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins


Author(s):  Li-na Yu, Jing Yu, Feng-jiang Zhang, Mei-juan Yang, Ting-ting Ding, Jun-kuan Wang, Wei He, Tao Fang, Gang Chen, Min Yan

Affiliation(s):  Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Anesthesiology, Jinhua People's Hospital, Jinhua 321300, China

Corresponding email(s):   yanminnina@hotmail.com

Key Words:  Sevoflurane, Postconditioning, Cardioprotection, Akt, Bcl-2, Bad


Li-na Yu, Jing Yu, Feng-jiang Zhang, Mei-juan Yang, Ting-ting Ding, Jun-kuan Wang, Wei He, Tao Fang, Gang Chen, Min Yan. Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins[J]. Journal of Zhejiang University Science B, 2010, 11(9): 661-672.

@article{title="Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins",
author="Li-na Yu, Jing Yu, Feng-jiang Zhang, Mei-juan Yang, Ting-ting Ding, Jun-kuan Wang, Wei He, Tao Fang, Gang Chen, Min Yan",
journal="Journal of Zhejiang University Science B",
volume="11",
number="9",
pages="661-672",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000155"
}

%0 Journal Article
%T Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins
%A Li-na Yu
%A Jing Yu
%A Feng-jiang Zhang
%A Mei-juan Yang
%A Ting-ting Ding
%A Jun-kuan Wang
%A Wei He
%A Tao Fang
%A Gang Chen
%A Min Yan
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 9
%P 661-672
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000155

TY - JOUR
T1 - Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins
A1 - Li-na Yu
A1 - Jing Yu
A1 - Feng-jiang Zhang
A1 - Mei-juan Yang
A1 - Ting-ting Ding
A1 - Jun-kuan Wang
A1 - Wei He
A1 - Tao Fang
A1 - Gang Chen
A1 - Min Yan
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 9
SP - 661
EP - 672
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000155


Abstract: 
sevoflurane postconditioning reduces myocardial infarct size. The objective of this study was to examine the role of the phosphatidylinositol-3-kinase (PI3K)/akt pathway in anesthetic postconditioning and to determine whether PI3K/akt signaling modulates the expression of pro- and antiapoptotic proteins in sevoflurane postconditioning. Isolated and perfused rat hearts were prepared first, and then randomly assigned to the following groups: Sham-operation (Sham), ischemia/reperfusion (Con), sevoflurane postconditioning (SPC), Sham plus 100 nmol/L wortmannin (Sham+Wort), Con+Wort, SPC+Wort, and Con+dimethylsulphoxide (DMSO). sevoflurane postconditioning was induced by administration of sevoflurane (2.5%, v/v) for 10 min from the onset of reperfusion. Left ventricular developed pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), maximum increase in rate of LVDP (+dP/dt), maximum decrease in rate of LVDP (−dP/dt), heart rate (HR), and coronary flow (CF) were measured at baseline, R30 min (30 min of reperfusion), R60 min, R90 min, and R120 min. Creatine kinase (CK) and lactate dehydrogenase (LDH) were measured after 5 min and 10 min reperfusion. Infarct size was determined by triphenyltetrazolium chloride staining at the end of reperfusion. Total akt and phosphorylated akt (phospho-akt), Bax, bcl-2, bad, and phospho-bad were determined by Western blot analysis. Analysis of variance (ANOVA) and Student-Newman-Keuls’ test were used to investigate the significance of differences between groups. The LVDP, ±dP/dt, and CF were higher and LVEDP was lower in the SPC group than in the Con group at all points of reperfusion (P<0.05). The SPC group had significantly reduced CK and LDH release and decreased infarct size compared with the Con group [(22.9±8)% vs. (42.4±9.4)%, respectively; P<0.05]. The SPC group also had increased the expression of phospho-akt, bcl-2, and phospho-bad, and decreased the expression of Bax. Wortmannin abolished the cardioprotection of sevoflurane postconditioning. sevoflurane postconditioning may protect the isolated rat heart. Activation of PI3K and modulation of the expression of pro- and antiapoptotic proteins may play an important role in sevoflurane-induced myocardial protection.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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