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Received: 2020-09-23

Revision Accepted: 2021-02-21

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Xingchi KAN


Yanling XU


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.11 P.929-940


Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway

Author(s):  Xingchi KAN, Yingsheng CHEN, Bingxu HUANG, Shoupeng FU, Wenjin GUO, Xin RAN, Yu CAO, Dianwen XU, Ji CHENG, Zhanqing YANG, Yanling XU

Affiliation(s):  Department of Theoretic Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun 130012, China; more

Corresponding email(s):   xuyanling0719@sina.com

Key Words:  Acute lung injury, Palrnatine, Lipopolysaccharide (LPS), Protein kinase B/nuclear factor-κ, B (Akt/NF-κ, B)

Xingchi KAN, Yingsheng CHEN, Bingxu HUANG, Shoupeng FU, Wenjin GUO, Xin RAN, Yu CAO, Dianwen XU, Ji CHENG, Zhanqing YANG, Yanling XU. Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway[J]. Journal of Zhejiang University Science B, 2021, 22(11): 929-940.

@article{title="Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway",
author="Xingchi KAN, Yingsheng CHEN, Bingxu HUANG, Shoupeng FU, Wenjin GUO, Xin RAN, Yu CAO, Dianwen XU, Ji CHENG, Zhanqing YANG, Yanling XU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway
%A Xingchi KAN
%A Yingsheng CHEN
%A Bingxu HUANG
%A Shoupeng FU
%A Wenjin GUO
%A Xin RAN
%A Dianwen XU
%A Zhanqing YANG
%A Yanling XU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 11
%P 929-940
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000583

T1 - Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway
A1 - Xingchi KAN
A1 - Yingsheng CHEN
A1 - Bingxu HUANG
A1 - Shoupeng FU
A1 - Wenjin GUO
A1 - Xin RAN
A1 - Yu CAO
A1 - Dianwen XU
A1 - Zhanqing YANG
A1 - Yanling XU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 11
SP - 929
EP - 940
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000583

Inflammation plays an important role in the development of acute lung injury (ALI). Severe pulmonary inflammation can cause acute respiratory distress syndrome (ARDS) or even death. Expression of proinflammatory interleukin-‍1β(IL-‍1β) and inducible nitric oxide synthase (iNOS) in the process of pulmonary inflammation will further exacerbate the severity of ALI. The purpose of this study was to explore the effect of palrnatine (Pa) on lipopolysaccharide (LPS)-induced mouse ALI and its underlying mechanism. Pa, a natural product, has a wide range of pharmacological activities with the potential to protect against lung injury. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed to detect the expression and translation of inflammatory genes and proteins in vitro and in vivo. Immunoprecipitation was used to detect the degree of P65 translocation into the nucleus. We also used molecular modeling to further clarify the mechanism of action. The results showed that Pa pretreatment could significantly inhibit the expression and secretion of the inflammatory cytokine IL-1β, and significantly reduce the protein level of the proinflammatory protease iNOS, in both in vivo and in vitro models induced by LPS. Further mechanism studies showed that Pa could significantly inhibit the activation of the protein kinase B (Akt)/nuclear factor-κB (NF-κb) signaling pathway in the LPS-induced ALI mode and in LPS-induced RAW264.7 cells. Through molecular dynamics simulation, we observed that Pa was bound to the catalytic pocket of Akt and effectively inhibited the biological activity of Akt. These results indicated that Pa significantly relieves LPS-induced ALI by activating the Akt/NF-κB signaling pathway.


方法:采用蛋白质印迹法(western blot)和实时荧光定量聚合酶链式反应(qRT-PCR)检测炎症基因和炎症蛋白在体内外的转录和翻译;使用免疫荧光法检测促炎转录因子核因子κB(NF-κB)P65转位进入细胞核程度;利用分子对接的方法模拟预测掌叶防己碱与Akt蛋白是否存在氢键作用。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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