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CLC number: Q819

On-line Access: 2011-11-04

Received: 2011-03-03

Revision Accepted: 2011-07-26

Crosschecked: 2011-10-17

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.11 P.931-934

http://doi.org/10.1631/jzus.B1100065


Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase


Author(s):  Sai-kun Pan, Sheng-jun Wu, Jin-moon Kim

Affiliation(s):  Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China, School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China, Food Safety and Inspection Service, Office of Field Operations, US Department of Agriculture, Albany, NY 12203, USA

Corresponding email(s):   jinkimnh@yahoo.com

Key Words:  Glucosamine, α, -Amylase, Glucoamylase, Hydrolysis


Sai-kun Pan, Sheng-jun Wu, Jin-moon Kim. Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase[J]. Journal of Zhejiang University Science B, 2011, 12(11): 931-934.

@article{title="Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase",
author="Sai-kun Pan, Sheng-jun Wu, Jin-moon Kim",
journal="Journal of Zhejiang University Science B",
volume="12",
number="11",
pages="931-934",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100065"
}

%0 Journal Article
%T Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase
%A Sai-kun Pan
%A Sheng-jun Wu
%A Jin-moon Kim
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 11
%P 931-934
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100065

TY - JOUR
T1 - Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase
A1 - Sai-kun Pan
A1 - Sheng-jun Wu
A1 - Jin-moon Kim
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 11
SP - 931
EP - 934
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100065


Abstract: 
Objective: In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. Methods: glucosamine was prepared by hydrolyzing chitosan, employing α;-Amylase initially, and subsequently, glucoamylase. Results: The optimal hydrolyzing conditions were as follows: reaction time, 4 h; pH, 5.0; temperature, 50 °C; and, α;-Amylase, 80 U/g for the initial reaction. Subsequently, glucoamylase was added in the presence of α;-Amylase. The optimal reaction conditions were found to be: reaction time, 8 h; pH, 4.5; temperature, 55 °C; and, glucoamylase, 4000 U/g. The hydrolysates were subject to filtrating, concentrating to about 20% (w/w), precipitating with five volumes of ethanol, and drying at 60 °C for 2 h. The content and the yield of glucosamine in the dried precipitate were 91.3% (w/w) and 86.2% (w/w), respectively. Conclusions: The method developed in this study is a promising option in the preparation of glucosamine.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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