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CLC number: Q7; Q5

On-line Access: 2014-05-05

Received: 2014-03-13

Revision Accepted: 2014-04-02

Crosschecked: 2014-04-16

Cited: 3

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.5 P.438-443


mRNA quality control at the 5' end*

Author(s):  Li-ting Zhai, Song Xiang

Affiliation(s):  . Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Corresponding email(s):   sxiang@sibs.ac.cn

Key Words:  mRNA capping, Quality control, Rai1, Dxo1, DXO

Li-ting Zhai, Song Xiang. mRNA quality control at the 5' end[J]. Journal of Zhejiang University Science B, 2014, 15(5): 438-443.

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T1 - mRNA quality control at the 5' end
A1 - Li-ting Zhai
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DOI - 10.1631/jzus.B1400070

All eukaryotic mRNAs are capped at their 5′ end. Capping of mRNAs takes place co-transcriptionally and involves three steps. The intermediates of the capping process, as well as the uncapped 5′ tri-phosphate RNA, are resistant to decapping and degradation by known factors, leading to the assumption that the capping process always proceeds to completion. This view was recently drastically changed. A novel family of enzymes, including the yeast proteins rai1, DXO1%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>DXO1/Ydr370C, and the mammalian protein DXO/Dom3Z, has been identified. These enzymes catalyze the conversion of the improperly capped mRNAs to 5′ mono-phosphate RNA, allowing them to be degraded by 5′-3′ exoribonucleases. Several of these enzymes also possess 5′-3′ exoribonuclease activities themselves, and can single-handedly clear the improperly capped mRNAs. Studying of these enzymes has led to the realization that mRNA capping does not always proceed to completion, and the identification of an mRNA capping quality control mechanism in eukaryotes. In this paper, we briefly review recent advances in this area.

mRNA 5\'端的质量控制

本文概要:所有真核生物的mRNA都在5'端被加帽,正确的加帽对mRNA的稳定性、出核及翻译调控具有重要意义。近年来,随着对Rai1及其同源蛋白的酶活性的发现,引出一个对mRNA加帽过程质量控制机制的发现。研究表明,Rai1及其同源蛋白可以将未被加帽(含5'端三磷酸基团)或未被正确加帽的mRNA转化成5'端含单磷酸基的RNA,使得它们可以被5'-3' RNA外切酶降解。某些Rai1的同源蛋白也具有5'-3' RNA外切酶的活性,可以同时完成降解RNA的工作。Rai1的同源蛋白在真核生物中广泛保守,提示这一机制普遍存在于真核生物中。

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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