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On-line Access: 2014-05-05

Received: 2014-03-13

Revision Accepted: 2014-04-02

Crosschecked: 2014-04-16

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.5 P.438-443

http://doi.org/10.1631/jzus.B1400070


mRNA quality control at the 5' end*


Author(s):  Li-ting Zhai, Song Xiang

Affiliation(s):  . Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Corresponding email(s):   sxiang@sibs.ac.cn

Key Words:  mRNA capping, Quality control, Rai1, Dxo1, DXO


Li-ting Zhai, Song Xiang. mRNA quality control at the 5' end[J]. Journal of Zhejiang University Science B, 2014, 15(5): 438-443.

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Abstract: 
All eukaryotic mRNAs are capped at their 5′ end. Capping of mRNAs takes place co-transcriptionally and involves three steps. The intermediates of the capping process, as well as the uncapped 5′ tri-phosphate RNA, are resistant to decapping and degradation by known factors, leading to the assumption that the capping process always proceeds to completion. This view was recently drastically changed. A novel family of enzymes, including the yeast proteins rai1, DXO1%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>DXO1/Ydr370C, and the mammalian protein DXO/Dom3Z, has been identified. These enzymes catalyze the conversion of the improperly capped mRNAs to 5′ mono-phosphate RNA, allowing them to be degraded by 5′-3′ exoribonucleases. Several of these enzymes also possess 5′-3′ exoribonuclease activities themselves, and can single-handedly clear the improperly capped mRNAs. Studying of these enzymes has led to the realization that mRNA capping does not always proceed to completion, and the identification of an mRNA capping quality control mechanism in eukaryotes. In this paper, we briefly review recent advances in this area.

mRNA 5\'端的质量控制

本文概要:所有真核生物的mRNA都在5'端被加帽,正确的加帽对mRNA的稳定性、出核及翻译调控具有重要意义。近年来,随着对Rai1及其同源蛋白的酶活性的发现,引出一个对mRNA加帽过程质量控制机制的发现。研究表明,Rai1及其同源蛋白可以将未被加帽(含5'端三磷酸基团)或未被正确加帽的mRNA转化成5'端含单磷酸基的RNA,使得它们可以被5'-3' RNA外切酶降解。某些Rai1的同源蛋白也具有5'-3' RNA外切酶的活性,可以同时完成降解RNA的工作。Rai1的同源蛋白在真核生物中广泛保守,提示这一机制普遍存在于真核生物中。
关键词:mRNA加帽;质量控制;Rai1;Dxo1;DXO

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

References

[1] Chang, J.H., Jiao, X., Chiba, K., 2012. Dxo1 is a new type of eukaryotic enzyme with both decapping and 5′-3′ exoribonuclease activity. Nat Struct Mol Biol, 19(10):1011-1017. 


[2] Coller, J., Parker, R., 2004. Eukaryotic mRNA decapping. Annu Rev Biochem, 73(1):861-890. 


[3] Cowling, V.H., 2010. Myc up-regulates formation of the mRNA methyl cap. Biochem Soc Trans, 38(6):1598-1601. 


[4] Cowling, V.H., Cole, M.D., 2007. The Myc transactivation domain promotes global phosphorylation of the RNA polymerase II carboxy-terminal domain independently of direct DNA binding. Mol Cell Biol, 27(6):2059-2073. 


[5] Cowling, V.H., Cole, M.D., 2010. Myc regulation of mRNA cap methylation. Genes Cancer, 1(6):576-579. 


[6] Dunckley, T., Parker, R., 1999. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif. EMBO J, 18(19):5411-5422. 


[7] Furuichi, Y., Shatkin, A.J., 2000. Viral and cellular mRNA capping: past and prospects. Adv Virus Res, 55:135-184. 


[8] Hocine, S., Singer, R.H., Grunwald, D., 2010. RNA processing and export. Cold Spring Harb Perspect Biol, 2(12):a000752


[9] Houseley, J., Tollervey, D., 2009. The many pathways of RNA degradation. Cell, 136(4):763-776. 


[10] Huh, W.K., Falvo, J.V., Gerke, L.C., 2003. Global analysis of protein localization in budding yeast. Nature, 425(6959):686-691. 


[11] Jiao, X., Xiang, S., Oh, C., 2010. Identification of a quality-control mechanism for mRNA 5′-end capping. Nature, 467(7315):608-611. 


[12] Jiao, X., Chang, J.H., Kilic, T., 2013. A mammalian pre-mRNA 5′ end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing. Mol Cell, 50(1):104-115. 


[13] Jimeno-Gonzalez, S., Haaning, L.L., Malagon, F., 2010. The yeast 5′-3′ exonuclease Rat1p functions during transcription elongation by RNA polymerase II. Mol Cell, 37(4):580-587. 


[14] Johnson, A.W., 1997. Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively. Mol Cell Biol, 17(10):6122-6130. 


[15] Kim, M., Krogan, N.J., Vasiljeva, L., 2004. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature, 432(7016):517-522. 


[16] Li, Y., Song, M., Kiledjian, M., 2011. Differential utilization of decapping enzymes in mammalian mRNA decay pathways. RNA, 17(3):419-428. 


[17] Lykke-Andersen, J., 2002. Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. Mol Cell Biol, 22(23):8114-8121. 


[18] Merrick, W.C., 2004. Cap-dependent and cap-independent translation in eukaryotic systems. Gene, 332:1-11. 


[19] Meyer, S., Temme, C., Wahle, E., 2004. Messenger RNA turnover in eukaryotes: pathways and enzymes. Crit Rev Biochem Mol Biol, 39(4):197-216. 


[20] Poole, T.L., Stevens, A., 1995. Comparison of features of the RNase activity of 5′-exonuclease-1 and 5′-exonuclease-2 of Saccharomyces cerevisiaeNucl Acids Symp Ser, 33:79-81. 

[21] Shatkin, A.J., 1976. Capping of eucaryotic mRNAs. Cell, 9(4):645-653. 


[22] Shuman, S., 1995. Capping enzyme in eukaryotic mRNA synthesis. Prog Nucl Acid Res Mol Biol, 50:101-129. 


[23] Song, M.G., Li, Y., Kiledjian, M., 2010. Multiple mRNA decapping enzymes in mammalian cells. Mol Cell, 40(3):423-432. 


[24] Stevens, A., 1978. An exoribonuclease from Saccharomyces cerevisiae: effect of modifications of 5′ end groups on the hydrolysis of substrates to 5′ mononucleotides. Biochem Biophys Res Commun, 81(2):656-661. 


[25] Stevens, A., Poole, T.L., 1995. 5′-Exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5′-exonuclease-1. J Biol Chem, 270(27):16063-16069. 


[26] Wang, Z., Jiao, X., Carr-Schmid, A., 2002. The hDcp2 protein is a mammalian mRNA decapping enzyme. PNAS, 99(20):12663-12668. 


[27] Wen, Y., Shatkin, A.J., 2000. Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-α. Genes Dev, 14(23):2944-2949. 


[28] Xiang, S., Cooper-Morgan, A., Jiao, X., 2009. Structure and function of the 5′→3′ exoribonuclease Rat1 and its activating partner Rai1. Nature, 458(7239):784-788. 


[29] Xue, Y., Bai, X., Lee, I., 2000.  Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol Cell Biol, 20(11):4006-4015. 


[30] Yang, W., 2011. Nucleases: diversity of structure, function and mechanism. Q Rev Biophys, 44(1):1-93. 


[31] Yue, Z., Maldonado, E., Pillutla, R., 1997. Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II. PNAS, 94(24):12898-12903. 


[32] Zheng, D., Chen, C.Y., Shyu, A.B., 2011. Unraveling regulation and new components of human P-bodies through a protein interaction framework and experimental validation. RNA, 17(9):1619-1634. 



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