CLC number: R735.9
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-04-15
Cited: 4
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Ri-sheng Que, Cheng Lin, Guo-ping Ding, Zheng-rong Wu, Li-ping Cao. Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer[J]. Journal of Zhejiang University Science B, 2016, 17(5): 352-360.
@article{title="Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer",
author="Ri-sheng Que, Cheng Lin, Guo-ping Ding, Zheng-rong Wu, Li-ping Cao",
journal="Journal of Zhejiang University Science B",
volume="17",
number="5",
pages="352-360",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500305"
}
%0 Journal Article
%T Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer
%A Ri-sheng Que
%A Cheng Lin
%A Guo-ping Ding
%A Zheng-rong Wu
%A Li-ping Cao
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 5
%P 352-360
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500305
TY - JOUR
T1 - Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer
A1 - Ri-sheng Que
A1 - Cheng Lin
A1 - Guo-ping Ding
A1 - Zheng-rong Wu
A1 - Li-ping Cao
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 5
SP - 352
EP - 360
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500305
Abstract: Background: Tumor-derived exosomes were considered to be potential candidates for tumor vaccines because they are abundant in immune-regulating proteins, whereas tumor exosomal miRNAs may induce immune tolerance, thereby having an opposite immune function. Objective: This study was designed to separate exosomal protein and depleted exosomal microRNAs (miRNAs), increasing the immune activity of exosomes for activating dendritic cell/cytokine-induced killer cells (DC/CIKs) against pancreatic cancer (PC). Methods: PC-derived exosomes (PEs) were extracted from cultured PANC-1 cell supernatants and then ruptured; this was followed by ultrafiltered exosome lysates (UELs). DCs were stimulated with lipopolysaccharide (LPS), PE, and UEL, followed by co-culture with CIKs. The anti-tumor effects of DC/CIKs against PC were evaluated by proliferation and killing rates, tumor necrosis factor-α (TNF-α) and perforin secretion. Exosomal miRNAs were depleted after lysis and ultrafiltration, while 128 proteins were retained, including several immune-activating proteins. Results: UEL-stimulated DC/CIKs showed a higher killing rate than LPS- and PE-stimulated DC/CIKs. Conclusions: miRNA-depleted exosome proteins may be promising agonists for specifically activating DC/CIKs against PC.
In this manuscript the authors study the potential value of miRNA-depleted exosomes for activation of DC/CIKs. The hypothesis that "PC derived exosomal miRNAs downregulate anti-tumor activities of DC/CIKs and depletion of exosomal miRNAs may enhance the anti-tumor activity of DC/CIKs" is well-reasoned and backed by previous research findings.
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[25]List of electronic supplementary materials
[26]Table S1 Proteomics identification of pancreatic cancer-derived ultrafiltered exosome lysates
[27]Fig. S1 Morphological and immunofluorescence characterization of DCs
[28]Fig. S2 Characterization of CIKs
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