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Journal of Zhejiang University SCIENCE A 2001 Vol.2 No.3 P.322-324

http://doi.org/10.1631/jzus.2001.0322


EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s


Author(s):  CAI Jian-ting, CHEN Peng, ZHONG Dan-dan, QIAN Ke-da

Affiliation(s):  Department of Gastroenterology, Second Affiliated Hospital, Collge of Medicine, Zhejiang University, Hangzhou 310009,China

Corresponding email(s): 

Key Words:  pancreatic carcinoma, antisense oligodeoxynucleotides, gene therapy, K-ras, concentration


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CAI Jian-ting, CHEN Peng, ZHONG Dan-dan, QIAN Ke-da. EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s[J]. Journal of Zhejiang University Science A, 2001, 2(3): 322-324.

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author="CAI Jian-ting, CHEN Peng, ZHONG Dan-dan, QIAN Ke-da",
journal="Journal of Zhejiang University Science A",
volume="2",
number="3",
pages="322-324",
year="2001",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0322"
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%T EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s
%A CAI Jian-ting
%A CHEN Peng
%A ZHONG Dan-dan
%A QIAN Ke-da
%J Journal of Zhejiang University SCIENCE A
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%P 322-324
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%I Zhejiang University Press & Springer
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T1 - EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s
A1 - CAI Jian-ting
A1 - CHEN Peng
A1 - ZHONG Dan-dan
A1 - QIAN Ke-da
J0 - Journal of Zhejiang University Science A
VL - 2
IS - 3
SP - 322
EP - 324
%@ 1869-1951
Y1 - 2001
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2001.0322


Abstract: 
Objective: To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Method: Human pancreatic cancer cell line PaTu8988s in exponential growth stage was used to study the effect of different drug concentrations on the cell line in the presence of different concentrations (0 μg/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out. Results: The inhibitory rate on the cell line PaTu8988s was 98.73%, 95.76%, 69.49%, 33.05% and 0 for ASODM concentrations of 200 μg/ml, 100 μg/ml, 50 μg/ml, 25 μg/ml and 5 μg/ml at 48 hours. Conclusions: k-ras complementary ASODN can inhibit the growth of human pancreatic cancer cell line PaTu8988s by 30.05% to 98.73%. This is likely to contribute to the specificity of the k-ras mRNA complementary capped ASODN sequential codon. Non-specific effect and side effect of ASOND were observed for instance, the greater the concentration is, the earlier the peak of inhibitory rate is reached. In concentration of 25 μg/ml to 100 μg/ml ASODN showed a dose-effect correlation.

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Reference

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