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CLC number: R783.1

On-line Access: 2016-11-03

Received: 2016-04-05

Revision Accepted: 2016-06-23

Crosschecked: 2016-10-18

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Christian Mehl


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.11 P.864-873


In vitro remineralization of hybrid layers using biomimetic analogs

Author(s):  Hui-ping Lin, Jun Lin, Juan Li, Jing-hong Xu, Christian Mehl

Affiliation(s):  Department of Stomatology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   junlin2@126.com, cmehl@proth.uni-kiel.de

Key Words:  Remineralization, Dentin, Adhesive resin, Biomimetic analog, Altered collagen, Confocal laser scanning microscopy (CLSM), Fluorescence

Hui-ping Lin, Jun Lin, Juan Li, Jing-hong Xu, Christian Mehl. In vitro remineralization of hybrid layers using biomimetic analogs[J]. Journal of Zhejiang University Science B, 2016, 17(11): 864-873.

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author="Hui-ping Lin, Jun Lin, Juan Li, Jing-hong Xu, Christian Mehl",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T In vitro remineralization of hybrid layers using biomimetic analogs
%A Hui-ping Lin
%A Jun Lin
%A Juan Li
%A Jing-hong Xu
%A Christian Mehl
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 11
%P 864-873
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600151

T1 - In vitro remineralization of hybrid layers using biomimetic analogs
A1 - Hui-ping Lin
A1 - Jun Lin
A1 - Juan Li
A1 - Jing-hong Xu
A1 - Christian Mehl
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 11
SP - 864
EP - 873
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600151

Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different adhesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premolars and bonded to composite resin using three adhesive resins (FluoroBond II, Xeno-III-Bond, and iBond). From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid (SBF). The experimental slabs were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs (biomineralization medium (BRM)). Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer (P≤0.05). A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.


目的:探讨再矿化介质聚乙烯磷酸和聚丙烯酸对3种粘结剂(FluoroBond II、Xeno-III和iBond)和牙本质所形成的树脂-牙本质界面再矿化程度的影响。
方法:将96颗健康前磨牙按照FluoroBond II、Xeno-III和iBond粘结剂随机分为3组,每颗牙均暴露表层牙本质。3种粘结剂分别严格按照各自产品说明书处理牙本质表面,牙合面堆制5 mm厚的树脂。每颗牙沿牙合-龈向切成0.9 mm厚的薄片,获取中间两片样本用于矿化组和模拟组。矿化组采用含聚乙烯磷酸和聚丙烯酸的再矿化液浸泡;模拟组采用不含聚乙烯磷酸和聚丙烯酸的模拟体液浸泡。各组标本在储存1、2、3和4个月后,各取出8片,经苏丹明B荧光染料染色24 h,冲洗,吹干,置CLSM下观察渗入混合层及粘结层的荧光情况,测量荧光面积、平均荧光量及总荧光量。所有数据统计方法采用方差分析(ANOVA)和Tukey’s HSD检验分析。
结论:本研究中聚丙烯酸和聚乙烯磷酸双仿生类似物分子对FluoroBond II、Xeno-III及iBond粘结剂均显示不同程度的再矿化效应,其中对iBond粘结剂再矿化效应最明显,FluoroBond II粘结剂次之,Xeno-III粘结剂再矿化效应较差,但能够起到抵制基底矿物继续丢失或阻止胶原降解的作用。CLSM结合应用苏丹明B是量化混合层再矿化的一项有效手段。因此,上述双仿生类似物分子应用于口腔粘结剂修复材料促使混合层再矿化,具有良好的应用前景,值得进一步深入研究。


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