CLC number: R542.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-11-10
Cited: 0
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Li-juan Shen, Shu Lu, Yong-hua Zhou, Lan Li, Qing-min Xing, Yong-liang Xu. Developing a rat model of dilated cardiomyopathy with improved survival[J]. Journal of Zhejiang University Science B, 2016, 17(12): 975-983.
@article{title="Developing a rat model of dilated cardiomyopathy with improved survival",
author="Li-juan Shen, Shu Lu, Yong-hua Zhou, Lan Li, Qing-min Xing, Yong-liang Xu",
journal="Journal of Zhejiang University Science B",
volume="17",
number="12",
pages="975-983",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600257"
}
%0 Journal Article
%T Developing a rat model of dilated cardiomyopathy with improved survival
%A Li-juan Shen
%A Shu Lu
%A Yong-hua Zhou
%A Lan Li
%A Qing-min Xing
%A Yong-liang Xu
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 12
%P 975-983
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600257
TY - JOUR
T1 - Developing a rat model of dilated cardiomyopathy with improved survival
A1 - Li-juan Shen
A1 - Shu Lu
A1 - Yong-hua Zhou
A1 - Lan Li
A1 - Qing-min Xing
A1 - Yong-liang Xu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 12
SP - 975
EP - 983
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600257
Abstract: To compare the continuous infusion and intermittent bolus injection administration protocols of doxorubicin (Dox) under the same cumulative dose (12 mg/kg), and establish a rat dilated cardiomyopathy model with improved survival, a total of 150 Sprague-Dawley (SD) rats were divided into three groups: a control group, administered with normal saline; a Dox 1 group, administration twice a week at 1 mg/kg; a Dox 2, administration once a week at 2 mg/kg. Mortality rates in the Dox 1 and Dox 2 groups were 22% and 48%, respectively (P<0.05). As shown by echocardiography, both Dox groups exhibited significant chamber dilatation and reduced cardiac function (all P<0.05 vs. control). Plasma brain natriuretic peptide and C-reactive protein concentrations were significantly increased (P<0.05) with both Dox regimens. The concentrations of Caspase-3 in myocardial tissues of rats significantly increased in both doxorubicin regimens. Myocardial metabolism imaging by histology and 18F-fluoro-deoxyglucose-positron emission tomography (18FDG-PET) both revealed decreased myocardial viability and necrosis, and even interstitial fibrosis, in left ventricles (LVs) in both Dox groups. Serum creatinine and aspartate aminotransferase concentrations were significantly higher in the Dox 2 model than in the Dox 1 model. doxorubicin given at both regimens induced dilated cardiomyopathy, while its administration at lower doses with more frequent infusions reduced the mortality rate.
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[37]List of electronic supplementary materials
[38]Fig. S1 Myocardial metabolism imaging by 18FDG-PET/CT
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