Full Text:   <2773>

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CLC number: R711.4

On-line Access: 2018-05-05

Received: 2017-03-08

Revision Accepted: 2017-06-25

Crosschecked: 2018-04-19

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Lei Yan


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.5 P.383-389


Creation of a rabbit model for intrauterine adhesions using electrothermal injury

Author(s):  Xin-xin Xu, Lian-bao Cao, Zhe Wang, Zhen Xu, Bing-qian Zhang, She-ling Wu, Sha-sha Qi, Lei Yan, Zi-jiang Chen

Affiliation(s):  Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University / National Research Center for Assisted Reproductive Technology and Reproductive Genetics / MOE Key Laboratory of Reproductive Endocrinology (Shandong University) / Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, China; more

Corresponding email(s):   yanleiandu@163.com

Key Words:  Intrauterine adhesion, Electrothermal injury, Rabbit model

Xin-xin Xu, Lian-bao Cao, Zhe Wang, Zhen Xu, Bing-qian Zhang, She-ling Wu, Sha-sha Qi, Lei Yan, Zi-jiang Chen. Creation of a rabbit model for intrauterine adhesions using electrothermal injury[J]. Journal of Zhejiang University Science B, 2018, 19(5): 383-389.

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author="Xin-xin Xu, Lian-bao Cao, Zhe Wang, Zhen Xu, Bing-qian Zhang, She-ling Wu, Sha-sha Qi, Lei Yan, Zi-jiang Chen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Creation of a rabbit model for intrauterine adhesions using electrothermal injury
%A Xin-xin Xu
%A Lian-bao Cao
%A Zhe Wang
%A Zhen Xu
%A Bing-qian Zhang
%A She-ling Wu
%A Sha-sha Qi
%A Lei Yan
%A Zi-jiang Chen
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 5
%P 383-389
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700086

T1 - Creation of a rabbit model for intrauterine adhesions using electrothermal injury
A1 - Xin-xin Xu
A1 - Lian-bao Cao
A1 - Zhe Wang
A1 - Zhen Xu
A1 - Bing-qian Zhang
A1 - She-ling Wu
A1 - Sha-sha Qi
A1 - Lei Yan
A1 - Zi-jiang Chen
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 5
SP - 383
EP - 389
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700086

The pathogenesis and therapeutic treatment of intrauterine adhesions (IUAs) remain unsolved, highlighting the need for stable and effective experimental animal models. In this study, uterine electrocoagulation of twenty-one female New Zealand White rabbits was carried out to establish an IUA model. As rabbits have two completely separate uterine horns, each rabbit had its own internal control: one uterine horn was given an electrothermal injury (Group A, n=21), and the contralateral uterine horn received no treatment and served as the control (Group B, n=21). The endometrial morphology, number of endometrial glands, area of endometrial fibrosis, and number of implanted fetuses were compared between the two groups. In Group A, the numbers of endometrial glands on Days 7 and 14 and the number of implanted fetuses were significantly lower than those in Group B (P<0.05, P<0.05, and P<0.01, respectively), while the ratio of the area with endometrial stromal fibrosis to the total endometrial area was significantly increased (P<0.01). These results suggest that this method of electrothermal injury is effective for the establishment of a rabbit IUA model between 7 and 14 d after surgery.


目的:目前宫腔粘连(Intrauterine adhesion,IUA)发病 机制及治疗方法仍未明确和统一,建立一个稳定有效的宫腔粘连动物模型是开展相关研究的前提和基础.本文旨在利用电热损伤法构建兔IUA模型,观察和评估该方法的建模效果.
方法:将21只成年雌性新西兰大白兔一侧子宫内膜用医用多功能高频电刀电灼损伤模拟宫腔粘连形成(A组,n=21),另一侧子宫不做处理作为自身对照(B组,n=21).分别在损伤后7、14和28天收集兔双侧子宫组织,行苏木精-伊红染色法(HE)和Masson染色观察两侧子宫内膜病理改变,并对两侧子宫内膜的腺体个数和内膜纤维化面积比进行统计学分析和比较.另外,将损伤后7天的雌兔与成年雄兔合笼, 14天后观察比较两侧子宫胚胎个数.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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