Full Text:   <1843>

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CLC number: R282.5

On-line Access: 2017-11-06

Received: 2016-06-24

Revision Accepted: 2016-11-10

Crosschecked: 2017-10-12

Cited: 0

Clicked: 3144

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yun-fei Ma

http://orcid.org/0000-0002-6176-3496

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.11 P.986-993

http://doi.org/10.1631/jzus.B1600289


Yimu San improves obstetric ability of pregnant mice by increasing serum oxytocin levels and connexin 43 expression in uterine smooth muscle


Author(s):  Qi-huan Wang, Shuang Zhang, Li-meng Qin, Wen-jun Zhang, Feng-hua Liu, Jian-qin Xu, Yun-fei Ma, Ke-dao Teng

Affiliation(s):  College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; more

Corresponding email(s):   yunfeima@cau.edu.cn, kedao@cau.edu.cn

Key Words:  Yimu San, Delivery ability, Uterine contraction, Oxytocin, Prostaglandin E2, Connexin 43


Qi-huan Wang, Shuang Zhang, Li-meng Qin, Wen-jun Zhang, Feng-hua Liu, Jian-qin Xu, Yun-fei Ma, Ke-dao Teng. Yimu San improves obstetric ability of pregnant mice by increasing serum oxytocin levels and connexin 43 expression in uterine smooth muscle[J]. Journal of Zhejiang University Science B, 2017, 18(11): 986-993.

@article{title="Yimu San improves obstetric ability of pregnant mice by increasing serum oxytocin levels and connexin 43 expression in uterine smooth muscle",
author="Qi-huan Wang, Shuang Zhang, Li-meng Qin, Wen-jun Zhang, Feng-hua Liu, Jian-qin Xu, Yun-fei Ma, Ke-dao Teng",
journal="Journal of Zhejiang University Science B",
volume="18",
number="11",
pages="986-993",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600289"
}

%0 Journal Article
%T Yimu San improves obstetric ability of pregnant mice by increasing serum oxytocin levels and connexin 43 expression in uterine smooth muscle
%A Qi-huan Wang
%A Shuang Zhang
%A Li-meng Qin
%A Wen-jun Zhang
%A Feng-hua Liu
%A Jian-qin Xu
%A Yun-fei Ma
%A Ke-dao Teng
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 11
%P 986-993
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600289

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T1 - Yimu San improves obstetric ability of pregnant mice by increasing serum oxytocin levels and connexin 43 expression in uterine smooth muscle
A1 - Qi-huan Wang
A1 - Shuang Zhang
A1 - Li-meng Qin
A1 - Wen-jun Zhang
A1 - Feng-hua Liu
A1 - Jian-qin Xu
A1 - Yun-fei Ma
A1 - Ke-dao Teng
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 11
SP - 986
EP - 993
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600289


Abstract: 
Prolonged farrowing remains one of the critical challenges in intensive pig farming. This study aims to explore the effects and mechanism of yimu San (YMS), a Chinese veterinary medicine micro mist, on delivery ability with mouse models. Thirty-two pregnant mice were randomly divided into a control group and low-YMS, med-YMS, and high-YMS groups. The labor process time and stillbirth rate were recorded, the levels of serum oxytocin and prostaglandin E2 (PGE2) were measured with enzyme-linked immunosorbent assay (ELISA). Contractility measurements of the isolated uterus and the expression of connexin 43 (Cx43) in uterine smooth muscle were evaluated. The results showed that compared with the control group, the birth process time and stillbirth rate in the med-YMS and high-YMS groups were remarkably lower. The in vitro uterine contractions, levels of oxytocin, PGE2, and Cx43 in the med-YMS and high-YMS groups were significantly higher than those in the control group. The differences of the above measurements between the low-YMS group and the control group were not obvious. It can be speculated that YMS could significantly promote labor in pregnant mice by enhancing the levels of oxytocin, Cx43, and PGE2.

益母散通过增加催产素水平和子宫平滑肌缝隙连接蛋白43的表达提高孕鼠分娩能力

目的:评价益母散对孕鼠分娩能力的促进作用,并初步探讨其作用机制。
创新点:首次在临近分娩小鼠中证明新中兽医药处方益母散可明显促进孕鼠分娩能力,且此作用与催产素和子宫平滑肌缝隙连接蛋白43(Cx43)的高表达相关。
方法:将妊娠小鼠分为对照组和益母散低、中、高剂量组,产前3天持续灌胃,统计孕鼠分娩产程和死胎率。采用离体平滑肌张力试验测定子宫平滑肌张力,用酶联免疫吸附测定(ELISA)方法检测血清中催产素和前列腺素E2(PGE2)含量,用蛋白免疫印迹(Western blotting)及免疫组织化学等方法检测小鼠子宫平滑肌中Cx43含量。
结论:益母散灌服处理能够使小鼠分娩产程缩短和死胎率降低(图1),并增强分娩小鼠子宫平滑肌张力(图2)。ELISA实验结果显示,益母散灌服处理使分娩小鼠血清催产素水平显著提高(图3)。免疫组化和免疫印迹实验结果显示,益母散灌服处理使分娩小鼠子宫平滑肌Cx43含量显著提高(图4和5)。综上所述,益母散具有提高孕鼠分娩能力的作用,该作用与催产素水平和子宫平滑肌Cx43的高表达相关。

关键词:益母散;催产素;前列腺素E2;缝隙连接蛋白43

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