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Received: 2016-12-03

Revision Accepted: 2017-03-03

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.12 P.1031-1045


Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro

Author(s):  Xi-han Guo, Juan Ni, Jing-lun Xue, Xu Wang

Affiliation(s):  School of Life Sciences, the Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming 650500, China; more

Corresponding email(s):   wangxu@fudan.edu.cn

Key Words:  Phyllanthus emblica, Mitomycin C, Cisplatin, Genomic instability, Cytokinesis-block micronucleus assay

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Xi-han Guo, Juan Ni, Jing-lun Xue, Xu Wang. Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro[J]. Journal of Zhejiang University Science B, 2017, 18(12): 1031-1045.

@article{title="Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro",
author="Xi-han Guo, Juan Ni, Jing-lun Xue, Xu Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro
%A Xi-han Guo
%A Juan Ni
%A Jing-lun Xue
%A Xu Wang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 12
%P 1031-1045
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600542

T1 - Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to normal cells in vitro
A1 - Xi-han Guo
A1 - Juan Ni
A1 - Jing-lun Xue
A1 - Xu Wang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 12
SP - 1031
EP - 1045
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600542

Objective: Fruit of Phyllanthus emblica Linn. (PE) is widely consumed as a functional food and used as a folk medicine due to its remarkable nutritional and pharmacological effects. mitomycin C (MMC) and cisplatin (cDDP) are the most widely used forms of chemotherapeutic drug, but their clinical use is limited by their genotoxicity to normal cells. We aimed to determine whether PE has potential to reduce the genotoxicity, while improving the anticancer effect, of MMC and cDDP. Methods: Cell proliferation was evaluated using the trypan blue exclusion assay and colony-forming assay. genomic instability (GIN) was measured using the cytokinesis-block micronucleus assay. Results: Co-treatment (72 h) with PE at 20–320 μg/ml significantly enhanced the efficacy of MMC (0.05 μg/ml) and cDDP (1 μg/ml) against Colo205 colorectal cancer cells (P<0.05), and at 80–320 μg/ml significantly decreased MMC- and cDDP-induced GIN and multinucleation in normal colonic NCM460 cells (P<0.05). PE significantly decreased the mitotic index (P<0.01), blocked mitotic progression (P<0.05), and promoted apoptosis (P<0.01) in MMC- and cDDP-treated NCM460 cells, suggesting that PE-mediated inhibition of mitosis and induction of apoptosis may limit the division and survival of highly damaged cells. Also, PE was found to inhibit the clonal expansion of MMC- and cDDP-treated NCM460 cells (P<0.05) and decrease the heterogeneity of the surviving clones. Conclusions: PE potentiates the anticancer efficacy of MMC and cDDP, while preventing their genotoxicity and inhibiting clonal expansions of unstable genomes in normal cells. These data suggest that PE has the potential to reduce the risk of secondary cancers induced by chemotherapeutics.


方法:人结肠癌Colo205细胞和人正常结肠上皮NCM460细胞分别经PE、PE+MMC组合或PE+cDDP组合处理72 h。细胞增殖用台盼蓝拒染法和克隆形成法测定,遗传毒性用胞质分裂阻断微核分析法(CBMN)测定。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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