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CLC number: Q812

On-line Access: 2018-08-02

Received: 2017-07-07

Revision Accepted: 2017-10-02

Crosschecked: 2018-07-10

Cited: 0

Clicked: 2792

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Chao-Yang Lin

https://orcid.org/0000-0002-4845-2220

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.610-619

http://doi.org/10.1631/jzus.B1700345


Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance


Author(s):  Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin

Affiliation(s):  Institute of Insect Science, Department of Plant Protection, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   chylin@zju.edu.cn

Key Words:  Transgenic maize, Bacillus thuringiensis (Bt), Insect resistance, Glyphosate tolerance


Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin. Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance[J]. Journal of Zhejiang University Science B, 2018, 19(8): 610-619.

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author="Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin",
journal="Journal of Zhejiang University Science B",
volume="19",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700345"
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%T Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
%A Miao-Miao Liu
%A Xiao-Jing Zhang
%A Yan Gao
%A Zhi-Cheng Shen
%A Chao-Yang Lin
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700345

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T1 - Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
A1 - Miao-Miao Liu
A1 - Xiao-Jing Zhang
A1 - Yan Gao
A1 - Zhi-Cheng Shen
A1 - Chao-Yang Lin
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700345


Abstract: 
A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein Cry1Ab/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein Cry1Ab/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostrinia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line.

抗虫抗草甘膦转基因玉米分子的特征及功能评价

目的:研究抗虫抗草甘膦玉米转化体ZD12-6外源基因的分子特征,对ZD12-6的抗虫和抗草甘膦性状进行综合评价,为ZD12-6产业化提供基础信息.
创新点:抗虫抗草甘膦转基因玉米ZD12-6是聚合了双抗虫基因和抗草甘膦基因的转化体,多基因聚合有利于后期品种转育和多性状叠加.对该转化体的分子特征信息分析和功能评价是评判其产业化价值的重要依据.
方法:利用DNA印迹法(Southern blot)研究外源基因插入拷贝数;利用高效热不对称交错聚合酶链反应(hiTAIL-PCR)方法对外源基因的插入位点进行定位;通过蛋白质印迹法(Western blot)对外源蛋白进行定性分析;利用酶联免疫吸附测定(ELISA)分析外源蛋白表达量;通过生物测定对抗虫和草甘膦的抗性水平进行评估.
结论:抗虫抗草甘膦玉米转化体ZD12-6中外源基因单拷贝插入,插入位点位于玉米1号染色体.ZD12-6对玉米上的主要害虫具有良好抗性,同时对草甘膦具有较强耐受性,具有产业化推广潜力.

关键词:转基因玉米;苏云金芽孢杆菌;抗虫;抗草甘膦

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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