Full Text:   <1197>

Summary:  <1181>

Suppl. Mater.: 

CLC number: R96

On-line Access: 2019-07-05

Received: 2018-12-29

Revision Accepted: 2019-04-04

Crosschecked: 2019-06-13

Cited: 0

Clicked: 2208

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yan-chun Hu

https://orcid.org/0000-0002-2998-7879

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.8 P.693-698

http://doi.org/10.1631/jzus.B1800645


Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice


Author(s):  Wei Sun, Chao-rong Zeng, Dong Yue, Yan-chun Hu

Affiliation(s):  Key Laboratory of Animal Disease and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):   hychun114@163.com

Key Words:  Ageratina adenophora, Hepatotoxicity, Mitochondrial dysfunction, ATPase, Mitochondrial DNA


Share this article to: More <<< Previous Article|

Wei Sun, Chao-rong Zeng, Dong Yue, Yan-chun Hu. Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice[J]. Journal of Zhejiang University Science B, 2019, 20(8): 693-698.

@article{title="Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice",
author="Wei Sun, Chao-rong Zeng, Dong Yue, Yan-chun Hu",
journal="Journal of Zhejiang University Science B",
volume="20",
number="8",
pages="693-698",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800645"
}

%0 Journal Article
%T Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice
%A Wei Sun
%A Chao-rong Zeng
%A Dong Yue
%A Yan-chun Hu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 8
%P 693-698
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800645

TY - JOUR
T1 - Involvement of mitochondrial dysfunction in hepatotoxicity induced by Ageratina adenophora in mice
A1 - Wei Sun
A1 - Chao-rong Zeng
A1 - Dong Yue
A1 - Yan-chun Hu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 8
SP - 693
EP - 698
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800645


Abstract: 
Ageratina adenophora is a noxious plant and it is known to cause acute asthma, diarrhea, depilation, and even death in livestock (Zhu et al., 2007; Wang et al., 2017). A. adenophora grows near roadsides and degraded land worldwide (He et al., 2015b). In the areas where it grows, A. adenophora is an invasive species that inhibits the growth of local plants and causes poisoning in animals that come in contact with it (Nie et al., 2012). In China, these plants can be found in Yunnan, Sichuan, Guizhou, Chongqing, and other southwestern areas (He et al., 2015a) and they have become a dominant species in these local regions. It threatens the native biodiversity and ecosystem in the invaded areas and causes serious economic losses (Wang et al., 2017). It has been reported that A. adenophora can grow in the northeast direction at a speed of 20 km per year in China (Guo et al., 2009). Because of the damage caused by A. adenophora, it ranks among the earliest alien invasive plant species in China (Wang et al., 2017).

紫茎泽兰引起小鼠线粒体功能障碍从而导致肝脏毒性损伤

目的:研究紫茎泽兰造成小鼠肝脏毒性损伤后,肝脏线粒体结构和功能发生变化的情况.
创新点:紫茎泽兰可以导致肝脏细胞发生凋亡和焦亡,从而产生中毒损伤,然而尚未有报道其对线粒体超微结构和功能的改变.本研究通过多种手段解决了这一问题.
方法:将40只小鼠随机分成4组,分别饲喂不同浓度的紫茎泽兰饲料(对照组、100、200和300 g/kg紫茎泽兰添加饲料组).采用苏木精-伊红染色法(H&E)研究肝脏损伤情况,利用透射电子显微技术研究线粒体超微结构改变.同时,结合实时荧光定量聚合酶链反应(qRT-PCR)、流式细胞术和化学分析方法对线粒体DNA拷贝数、肿胀度和三磷酸腺苷(ATP)酶活性的改变进行探究.
结论:紫茎泽兰导致线粒体超微结构的改变,增大了线粒体肿胀度,降低了钠钾ATP酶(Na+K+-ATPase)和钙镁ATP酶(Ca2+Mg2+-ATPase)活力,同时减少了DNA拷贝数,从而引起肝脏损伤.

关键词:紫茎泽兰;肝毒性;线粒体功能障碍;三磷酸腺苷(ATP)酶;线粒体DNA

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Guo CX, Wang J, Jing L, et al., 2018. Mitochondrial dysfunction, perturbations of mitochondrial dynamics and biogenesis involved in endothelial injury induced by silica nanoparticles. Environ Pollut, 236:926-936.

[2]Guo SH, Li W, Zhang LB, et al., 2009. Kinetics and equilibrium adsorption study of lead(II) onto the low cost adsorbent— Eupatorium adenophorum spreng. Process Saf Environ Prot, 87(5):343-351.

[3]He YJ, Chen WH, Hu YC, et al., 2015a. E. adenophorum induces cell cycle and apoptosis of renal cells through mitochondrial pathway and caspase activation in Saanen goat. PLoS ONE, 10(9):e0138504.

[4]He YJ, Mo Q, Hu YC, et al., 2015b. E. adenophorum induces cell cycle arrest and apoptosis of splenocytes through the mitochondrial pathway and caspase activation in Saanen goats. Sci Rep, 5:15967.

[5]He YJ, Mo Q, Luo B, et al., 2016. Induction of apoptosis and autophagy via mitochondria- and PI3K/Akt/mTOR-mediated pathways by E. adenophorum in hepatocytes of Saanen goat. Oncotarget, 7(34):54537-54548.

[6]https://doi.org/10.18632/oncotarget.10402

[7]Katoch R, Sharma OP, Dawra RK, et al., 2000. Hepatotoxicity of Eupatorium adenophorum to rats. Toxicon, 38(2):309-314.

[8]Kaushal V, Dawra RK, Sharma OP, et al., 2001. Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum. Vet Res Commun, 25(7):601-608.

[9]Koh H, Park GS, Shin SM, et al., 2018. Mitochondrial mutations in cholestatic liver disease with biliary atresia. Sci Rep, 8:905.

[10]Larosche I, Lettéron P, Berson A, et al., 2010. Hepatic mitochondrial DNA depletion after an alcohol binge in mice: probable role of peroxynitrite and modulation by manganese superoxide dismutase. J Pharmacol Exp Ther, 332(3):886-897.

[11]Nie XJ, Lv SZ, Zhang YX, et al., 2012. Complete chloroplast genome sequence of a major invasive species, Crofton weed (Ageratina adenophora). PLoS ONE, 7(5):e36869.

[12]Nugent SME, Mothersill CE, Seymour C, et al., 2007. Increased mitochondrial mass in cells with functionally compromised mitochondria after exposure to both direct γ radiation and bystander factors. Radiat Res, 168(1):134-142.

[13]O'Sullivan BM, 1985. Investigations into Crofton weed (Eupatorium adenophorum) toxicity in horses. Aust Vet J, 62(1):30-32.

[14]Robin ED, Wong R, 1988. Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells. J Cell Physiol, 136(3):507-513.

[15]Sani Y, Harper PAW, Cook RL, et al., 1992. The toxicity of Eupatorium adenophorum for the liver of the mouse. Proceedings of the Third International Symposium. Iowa State University Press, USA, p.626-629.

[16]Shay JW, Pierce DJ, Werbin H, 1990. Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions. J Biol Chem, 265(25):14802-14807.

[17]Shi XX, Bai HY, Zhao M, et al., 2018. Treatment of acetaminophen-induced liver injury with exogenous mitochondria in mice. Transl Res, 196:31-41.

[18]Song Y, Wu ZC, Ding W, et al., 2018. NF-κB in mitochondria regulates PC12 cell apoptosis following lipopolysaccharide-induced injury. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 19(6):425-435.

[19]Sun W, Zeng CR, Liu SS, et al., 2018. Ageratina adenophora induces mice hepatotoxicity via ROS-NLRP3-mediated pyroptosis. Sci Rep, 8:16032.

[20]Taruno A, 2018. ATP release channels. Int J Mol Sci, 19(3):808.

[21]Tiao MM, Lin TK, Kuo FY, et al., 2007. Early stage of biliary atresia is associated with significant changes in 8-hydroxydeoxyguanosine and mitochondrial copy number. J Pediatr Gastroenterol Nutr, 45(3):329-334.

[22]Wang C, Lin HL, Feng QS, et al., 2017. A new strategy for the prevention and control of Eupatorium adenophorum under climate change in China. Sustainability, 9(11):2037.

[23]Wang LY, Wang DH, Zou XY, et al., 2009. Mitochondrial functions on oocytes and preimplantation embryos. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 10(7):483-492.

[24]Yang HJ, Du RW, Wu YK, et al., 2017. Microbial composting and detoxification of Ageratina adenophora. Acta Pratacult Sin, 26(6):131-138 (in Chinese).

[25]Zhu L, Sun OJ, Sang WG, et al., 2007. Predicting the spatial distribution of an invasive plant species (Eupatorium adenophorum) in China. Landscape Ecol, 22(8):1143-1154.

[26]List of electronic supplementary materials

[27]Data S1 Materials and methods

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE