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CLC number: R592

On-line Access: 2020-04-07

Received: 2020-06-05

Revision Accepted: 2020-03-11

Crosschecked: 2020-03-11

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.4 P.291-304


Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology

Author(s):  Jia Xu, Jiu-Kun Jiang, Xiao-Lin Li, Xiao-Peng Yu, Ying-Ge Xu, Yuan-Qiang Lu

Affiliation(s):  Department of Emergency Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   luyuanqiang@zju.edu.cn

Key Words:  Human umbilical vein endothelial cells (HUVECs), Hypoxia, Re-oxygenation, Microarray, Pleckstrin homology-like domain family A member 1 (PHLDA1), Long non-coding RNA (lncRNA)

Jia Xu, Jiu-Kun Jiang, Xiao-Lin Li, Xiao-Peng Yu, Ying-Ge Xu, Yuan-Qiang Lu. Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology[J]. Journal of Zhejiang University Science B, 2020, 21(4): 291-304.

@article{title="Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology",
author="Jia Xu, Jiu-Kun Jiang, Xiao-Lin Li, Xiao-Peng Yu, Ying-Ge Xu, Yuan-Qiang Lu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology
%A Jia Xu
%A Jiu-Kun Jiang
%A Xiao-Lin Li
%A Xiao-Peng Yu
%A Ying-Ge Xu
%A Yuan-Qiang Lu
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 4
%P 291-304
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000043

T1 - Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology
A1 - Jia Xu
A1 - Jiu-Kun Jiang
A1 - Xiao-Lin Li
A1 - Xiao-Peng Yu
A1 - Ying-Ge Xu
A1 - Yuan-Qiang Lu
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 4
SP - 291
EP - 304
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000043

Objective: To provide comprehensive data to understand mechanisms of vascular endothelial cell (VEC) response to hypoxia/re-oxygenation. Methods: human umbilical vein endothelial cells (HUVECs) were employed to construct hypoxia/re-oxygenation-induced VEC transcriptome profiling. Cells incubated under 5% O2, 5% CO2, and 90% N2 for 3 h followed by 95% air and 5% CO2 for 1 h were used in the hypoxia/re-oxygenation group. Those incubated only under 95% air and 5% CO2 were used in the normoxia control group. Results: By using a well-established microarray chip consisting of 58 339 probes, the study identified 372 differentially expressed genes. While part of the genes are known to be VEC hypoxia/re-oxygenation-related, serving as a good control, a large number of genes related to VEC hypoxia/re-oxygenation were identified for the first time. Through bioinformatic analysis of these genes, we identified that multiple pathways were involved in the reaction. Subsequently, we applied real-time polymerase chain reaction (PCR) and western blot techniques to validate the microarray data. It was found that the expression of apoptosis-related proteins, like pleckstrin homology-like domain family A member 1 (PHLDA1), was also consistently up-regulated in the hypoxia/re-oxygenation group. STRING analysis found that significantly differentially expressed genes SLC38A3, SLC5A5, Lnc-SLC36A4-1, and Lnc-PLEKHJ1-1 may have physical or/and functional protein–protein interactions with PHLDA1. Conclusions: The data from this study have built a foundation to develop many hypotheses to further explore the hypoxia/re-oxygenation mechanisms, an area with great clinical significance for multiple diseases.


方法:采用缺氧孵育3 h后复氧1 h的HUVEC为缺氧/复氧组,同时常氧孵育的HUVEC为常氧对照组.应用含58 339条探针的全转录组芯片检测每组三个样本.对差异表达基因进行生信分析和功能验证.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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