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Lei XU


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.4 P.285-294


High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition

Author(s):  Lei XU, Zhifang WU, Yuan WANG, Sa WANG, Chang SHU, Zhuhui DUAN, Shuli DENG

Affiliation(s):  The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China; more

Corresponding email(s):   dengshuli@zju.edu.cn

Key Words:  Salivary microbiota, Caries-free, Preschool children, Primary dentition, Illumina MiSeq, 16S rDNA V3-V4 hypervariable regions

Lei XU, Zhifang WU, Yuan WANG, Sa WANG, Chang SHU, Zhuhui DUAN, Shuli DENG. High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition[J]. Journal of Zhejiang University Science B, 2021, 22(4): 285-294.

@article{title="High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition",
author="Lei XU, Zhifang WU, Yuan WANG, Sa WANG, Chang SHU, Zhuhui DUAN, Shuli DENG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition
%A Lei XU
%A Zhifang WU
%A Yuan WANG
%A Chang SHU
%A Zhuhui DUAN
%A Shuli DENG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 4
%P 285-294
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000554

T1 - High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition
A1 - Lei XU
A1 - Zhifang WU
A1 - Yuan WANG
A1 - Sa WANG
A1 - Chang SHU
A1 - Zhuhui DUAN
A1 - Shuli DENG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 4
SP - 285
EP - 294
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000554

ObjectivesThe study aimed at identifying salivary microbiota in caries-free Chinese preschool children using high-throughput sequencing.
MethodsSaliva samples were obtained from 35 caries-free preschool children (18 boys and 17 girls) with primary dentition, and 16S ribosomal DNA (rDNA) V3–V4 hypervariable regions of the microorganisms were analyzed using illumina MiSeq.
ResultsAt 97% similarity level, all of these reads were clustered into 334 operational taxonomic units (OTUs). Among these, five phyla (Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Candidate division TM7) and 13 genera (Streptococcus, Rothia, Granulicatella, Prevotella, Enterobacter, Veillonella, Neisseria, Staphylococcus, Janthinobacterium, Pseudomonas, Brevundimonas, Devosia, and Gemella) were the most dominant, constituting 99.4% and 89.9% of the salivary microbiota, respectively. The core salivary microbiome comprised nine genera (Actinomyces, Capnocytophaga, Gemella, Granulicatella, Lachnoanaerobaculum, Neisseria, Porphyromonas, Rothia, and Streptococcus). Analysis of microbial diversity and community structure revealed a similar pattern between male and female subjects. The difference in microbial community composition between them was mainly attributed to Neisseria (P=0.023). Furthermore, functional prediction revealed that the most abundant genes were related to amino acid transport and metabolism.
ConclusionsOur results revealed the diversity and composition of salivary microbiota in caries-free preschool children, with little difference between male and female subjects. Identity of the core microbiome, coupled with prediction of gene function, deepens our understanding of oral microbiota in caries-free populations and provides basic information for associating salivary microecology and oral health.


方法:我们采集了来自35名无龋学龄前乳牙列儿童的口腔唾液样本,其中包括18名男孩和17名女孩。利用Illumina MiSeq平台对微生物样本的16S rDNA V3-V4高变区进行测序分析,从而分析获得的微生物群落特征。

关键词:唾液微生物群落;无龋;学龄前儿童;乳牙列;MiSeq测序;16S rDNA V3-V4高变区

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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