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On-line Access: 2022-01-12

Received: 2021-06-10

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Feifei LI


Kehai LIU


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.1 P.84-88


Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages

Author(s):  Feifei LI, Kehai LIU, Kewu LIU

Affiliation(s):  College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; more

Corresponding email(s):   khliu@shou.edu.cn

Key Words:  Gracilaria chouae polysaccharide, Purification, Characterization, Immunological activity

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Feifei LI, Kehai LIU, Kewu LIU. Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages[J]. Journal of Zhejiang University Science B, 2022, 23(1): 84-88.

@article{title="Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages",
author="Feifei LI, Kehai LIU, Kewu LIU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages
%A Feifei LI
%A Kehai LIU
%A Kewu LIU
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 1
%P 84-88
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100508

T1 - Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages
A1 - Feifei LI
A1 - Kehai LIU
A1 - Kewu LIU
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 1
SP - 84
EP - 88
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100508

This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. GCP-3A was composed of xylose, galactose, glucose, and mannose with a molar ratio of 3.00:29.28:0.63:0.45, and it contained α,β‍-glycosidic linkages. Scanning electron microscopy (SEM) and a Congo red test showed that it was a heterogeneous polysaccharide with irregular interwoven sheets and rods, and did not have a triple-helix conform‍ation. Furthermore, GCP-3A significantly promoted the proliferation of RAW264.7 macrophages and the secretion of nitric oxide (NO) in tests of 3-‍(4,‍5-dimethylthiahiazo-2-yl)‍-2,‍5-diphenytetrazoliumromide(MTT) and NO.


结论:GCP-3A是一种硫酸化酸性多糖,分子量为11.87 kDa,由木糖、半乳糖、葡萄糖和甘露糖组成,四者的摩尔比为3.00:29.28:0.63:0.45,且含有α,β-糖苷键。GCP-3A是一种由不规则片状和棒状交织而成的异质多糖,无三螺旋构象。此外,GCP-3A显著性地促进了RAW264.7巨噬细胞的增殖和NO分泌,具有应用于医药及功能性食品的潜力。


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