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On-line Access: 2022-01-12

Received: 2021-06-10

Revision Accepted: 2021-08-23

Crosschecked: 0000-00-00

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Feifei LI

https://orcid.org/0000-0001-8743-6648

Kehai LIU

https://orcid.org/0000-0003-2505-5553

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.1 P.84-88

http://doi.org/10.1631/jzus.B2100508


Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages


Author(s):  Feifei LI, Kehai LIU, Kewu LIU

Affiliation(s):  College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; more

Corresponding email(s):   khliu@shou.edu.cn

Key Words:  Gracilaria chouae polysaccharide, Purification, Characterization, Immunological activity


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Feifei LI, Kehai LIU, Kewu LIU. Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages[J]. Journal of Zhejiang University Science B, 2022, 23(1): 84-88.

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author="Feifei LI, Kehai LIU, Kewu LIU",
journal="Journal of Zhejiang University Science B",
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pages="84-88",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100508"
}

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%A Feifei LI
%A Kehai LIU
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T1 - Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2100508


Abstract: 
This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. GCP-3A was composed of xylose, galactose, glucose, and mannose with a molar ratio of 3.00:29.28:0.63:0.45, and it contained α,β‍-glycosidic linkages. Scanning electron microscopy (SEM) and a Congo red test showed that it was a heterogeneous polysaccharide with irregular interwoven sheets and rods, and did not have a triple-helix conform‍ation. Furthermore, GCP-3A significantly promoted the proliferation of RAW264.7 macrophages and the secretion of nitric oxide (NO) in tests of 3-‍(4,‍5-dimethylthiahiazo-2-yl)‍-2,‍5-diphenytetrazoliumromide(MTT) and NO.

脆江蓠硫酸化多糖的化学表征及其对RAW264.7巨噬细胞的激活作用

目的:脆江蓠是中国沿海广泛分布的重要经济红藻,多糖是其主要生物成分,通过对其多糖分离纯化、化学表征及活性评价,为脆江蓠的开发奠定实验基础。
创新点:首次从脆江蓠中得到均一硫酸化多糖GCP-3A,并发现其多糖对巨噬细胞RAW264.7具有显著激活作用。
方法:以脆江蓠为原料,采用水提醇沉、柱层析等方法分离纯化得到脆江蓠硫酸化多糖GCP-3A;采用凝胶渗透色谱(GPC)、气相色谱-质谱(GC-MS)、傅里叶变换红外光谱(FT-IR)和核磁共振(NMR)探究其化学特征;采用扫描电镜(SEM)和刚果红研究其表面形态;通过噻唑蓝(MTT)和一氧化氮(NO)试验分析其对RAW264.7巨噬细胞的活化作用。
结论:GCP-3A是一种硫酸化酸性多糖,分子量为11.87 kDa,由木糖、半乳糖、葡萄糖和甘露糖组成,四者的摩尔比为3.00:29.28:0.63:0.45,且含有α,β-糖苷键。GCP-3A是一种由不规则片状和棒状交织而成的异质多糖,无三螺旋构象。此外,GCP-3A显著性地促进了RAW264.7巨噬细胞的增殖和NO分泌,具有应用于医药及功能性食品的潜力。

关键词:脆江蓠多糖;化学表征;巨噬细胞激活

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