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Received: 2005-10-29

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.3 P.186-192


Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31

Author(s):  Gao Jie, Bao Hua-Ying, Xin Ming-Xiu, Liu Yuan-Xia, Li Qian, Zhang Yan-Fen

Affiliation(s):  School of Chemistry, Beijing Normal University, Beijing 100875, China; more

Corresponding email(s):   hybao@bnu.edu.cn

Key Words:  Vagococcus sp., Bioflocculant, Characterization, Flocculating activity

Gao Jie, Bao Hua-Ying, Xin Ming-Xiu, Liu Yuan-Xia, Li Qian, Zhang Yan-Fen. Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31[J]. Journal of Zhejiang University Science B, 2006, 7(3): 186-192.

@article{title="Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31",
author="Gao Jie, Bao Hua-Ying, Xin Ming-Xiu, Liu Yuan-Xia, Li Qian, Zhang Yan-Fen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31
%A Gao Jie
%A Bao Hua-Ying
%A Xin Ming-Xiu
%A Liu Yuan-Xia
%A Li Qian
%A Zhang Yan-Fen
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 3
%P 186-192
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0186

T1 - Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31
A1 - Gao Jie
A1 - Bao Hua-Ying
A1 - Xin Ming-Xiu
A1 - Liu Yuan-Xia
A1 - Li Qian
A1 - Zhang Yan-Fen
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 3
SP - 186
EP - 192
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0186

Screening of microorganisms producing flocculating substances was carried out. A strain secreting a large amount of bioflocculant was isolated from wastewater samples collected from the Little Moon River in Beijing. Based on the morphological properties and 16S rDNA sequence analysis, the isolate (designated W31) was classified as Vagococcus sp. A bioflocculant (named MBFW31) produced by W31 was extracted from the culture broth by ethanol precipitation and purified by gel chromatography. MBFW31 was heat-stable and had strong flocculating activity in a wide range of pH with relatively low dosage requirement. MBFW31 was identified as a polysaccharide with molecular weight over 2×106. It contained neutral sugar and uronic acid as its major and minor components, respectively. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl group in its molecules. The present results suggested that MBFW31 had potential application in wastewater treatment.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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