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On-line Access: 2022-05-13

Received: 2021-11-12

Revision Accepted: 2022-02-04

Crosschecked: 2022-05-13

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Shisheng SUN


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.5 P.407-422


Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells

Author(s):  Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN

Affiliation(s):  College of Life Science, Northwest University, Xi’an 710069, China

Corresponding email(s):   suns@nwu.edu.cn

Key Words:  Macrophage, THP-1 cells, M2 subtype, Proteomics, Biological function

Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN. Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells[J]. Journal of Zhejiang University Science B, 2022, 23(5): 407-422.

@article{title="Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells",
author="Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells
%A Pengfei LI
%A Chen MA
%A Jing LI
%A Shanshan YOU
%A Liuyi DANG
%A Jingyu WU
%A Zhifang HAO
%A Jun LI
%A Yuan ZHI
%A Shisheng SUN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 5
%P 407-422
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100930

T1 - Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells
A1 - Pengfei LI
A1 - Chen MA
A1 - Jing LI
A1 - Shanshan YOU
A1 - Liuyi DANG
A1 - Jingyu WU
A1 - Zhifang HAO
A1 - Jun LI
A1 - Yuan ZHI
A1 - Lin CHEN
A1 - Shisheng SUN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 5
SP - 407
EP - 422
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100930

macrophages are widely distributed immune cells that contribute to tissue homeostasis. Human THP-1 cells have been widely used in various macrophage-associated studies, especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes. However, the molecular characterization of four m2 subtypes (M2a, M2b, M2c, and M2d) derived from THP-1 has not been fully investigated. In this study, we systematically analyzed the protein expression profiles of human THP-1-derived macrophages (M0, M1, M2a, M2b, M2c, and M2d) using quantitative proteomics approaches. The commonly and specially regulated proteins of the four m2 subtypes and their potential biological functions were further investigated. The results showed that M2a and M2b, and M2c and M2d have very similar protein expression profiles. These data could serve as an important resource for studies of macrophages using THP-1 cells, and provide a reference to distinguish different m2 subtypes in macrophage-associated diseases for subsequent clinical research.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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