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On-line Access: 2022-05-13

Received: 2021-11-12

Revision Accepted: 2022-02-04

Crosschecked: 2022-05-13

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Shisheng SUN

https://orcid.org/0000-0002-7242-7164

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.5 P.407-422

http://doi.org/10.1631/jzus.B2100930


Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells


Author(s):  Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN

Affiliation(s):  College of Life Science, Northwest University, Xi’an 710069, China

Corresponding email(s):   suns@nwu.edu.cn

Key Words:  Macrophage, THP-1 cells, M2 subtype, Proteomics, Biological function


Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN. Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells[J]. Journal of Zhejiang University Science B, 2022, 23(5): 407-422.

@article{title="Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells",
author="Pengfei LI, Chen MA, Jing LI, Shanshan YOU, Liuyi DANG, Jingyu WU, Zhifang HAO, Jun LI, Yuan ZHI, Lin CHEN, Shisheng SUN",
journal="Journal of Zhejiang University Science B",
volume="23",
number="5",
pages="407-422",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100930"
}

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%T Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells
%A Pengfei LI
%A Chen MA
%A Jing LI
%A Shanshan YOU
%A Liuyi DANG
%A Jingyu WU
%A Zhifang HAO
%A Jun LI
%A Yuan ZHI
%A Lin CHEN
%A Shisheng SUN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 5
%P 407-422
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100930

TY - JOUR
T1 - Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells
A1 - Pengfei LI
A1 - Chen MA
A1 - Jing LI
A1 - Shanshan YOU
A1 - Liuyi DANG
A1 - Jingyu WU
A1 - Zhifang HAO
A1 - Jun LI
A1 - Yuan ZHI
A1 - Lin CHEN
A1 - Shisheng SUN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 5
SP - 407
EP - 422
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100930


Abstract: 
macrophages are widely distributed immune cells that contribute to tissue homeostasis. Human THP-1 cells have been widely used in various macrophage-associated studies, especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes. However, the molecular characterization of four m2 subtypes (M2a, M2b, M2c, and M2d) derived from THP-1 has not been fully investigated. In this study, we systematically analyzed the protein expression profiles of human THP-1-derived macrophages (M0, M1, M2a, M2b, M2c, and M2d) using quantitative proteomics approaches. The commonly and specially regulated proteins of the four m2 subtypes and their potential biological functions were further investigated. The results showed that M2a and M2b, and M2c and M2d have very similar protein expression profiles. These data could serve as an important resource for studies of macrophages using THP-1 cells, and provide a reference to distinguish different m2 subtypes in macrophage-associated diseases for subsequent clinical research.

人THP-1来源的四种M2巨噬细胞亚型的蛋白质组学特征

目的:探究THP-1来源的四种M2巨噬细胞亚型的蛋白质组学特征,为进一步区分M2亚型以及使用THP-1来源的巨噬细胞作为临床前研究的细胞模型提供数据基础。
创新点:首次系统性地对四种M2巨噬细胞亚型的蛋白质组进行了分析,并提供潜在的候选标志物用于不同M2亚型的鉴定,为巨噬细胞的可塑性、相关疾病以及免疫机制等方面的研究提供参考。
方法:在体外诱导THP-1单核细胞分化为M0、M1、M2a、M2b、M2c和M2d巨噬细胞,并利用基于质谱的无标记定量技术揭示了其蛋白质组学特征。结合数据库与生物信息学分析四种M2亚型共同或特异的生物学功能及疾病关联。此外,通过靶向蛋白质组学技术分析了四种M2巨噬细胞亚型中共同或特异上调的蛋白。
结论:本研究首次揭示了THP-1衍生的四种M2巨噬细胞亚型的蛋白质组学特征。不仅验证了几个已知的巨噬细胞标记物,也提供了一份新的候选标记物列表,用于识别M2a、M2b、M2c和M2d巨噬细胞。一个主要发现是M2a和M2c以及M2b和M2d的蛋白表达谱非常相似。本研究的数据为未来研究巨噬细胞相关疾病的免疫调节机制以及使用THP-1来源的巨噬细胞模型进行临床前研究提供了宝贵的资源。

关键词:巨噬细胞;THP-1细胞;M2亚型;蛋白质组学;生物学功能

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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