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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.10 P.922-934


Cellulose nanofibril matrix drives the dynamic formation of spheroids

Author(s):  Yi LU, Guo LI, Yeqiu LI, Yuan YAO

Affiliation(s):  College of Chemical and Biological Engineering, Zhejiang University,Hangzhou310027,China; more

Corresponding email(s):   yyao1@zju.edu.cn

Key Words:  Cellulose, Nanofibril, Matrix, Self-assembly, Spheroid

Yi LU, Guo LI, Yeqiu LI, Yuan YAO. Cellulose nanofibril matrix drives the dynamic formation of spheroids[J]. Journal of Zhejiang University Science B, 2023, 24(10): 922-934.

@article{title="Cellulose nanofibril matrix drives the dynamic formation of spheroids",
author="Yi LU, Guo LI, Yeqiu LI, Yuan YAO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cellulose nanofibril matrix drives the dynamic formation of spheroids
%A Yi LU
%A Guo LI
%A Yeqiu LI
%A Yuan YAO
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 10
%P 922-934
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B23d0003

T1 - Cellulose nanofibril matrix drives the dynamic formation of spheroids
A1 - Yi LU
A1 - Guo LI
A1 - Yeqiu LI
A1 - Yuan YAO
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 10
SP - 922
EP - 934
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B23d0003

Multicellular spheroids, which mimic the natural organ counterparts, allow the prospect of drug screening and regenerative medicine. However, their application is hampered by low processing efficiency or limited scale. This study introduces an efficient method to drive rapid multicellular spheroid formation by a cellulose nanofibril matrix. This matrix enables the facilitated growth of spheroids (within 48 h) through multiple cell assembly into size-controllable aggregates with well-organized physiological microstructure. The efficiency, dimension, and conformation of the as-formed spheroids depend on the concentration of extracellular nanofibrils, the number of assembled cells, and the heterogeneity of cell types. The above strategy allows the robust formation mechanism of compacted tumoroids and hepatocyte spheroids.


卢轶1,2,3, 李果1,2, 李叶秋2, 姚远1,2,3
1浙江大学化学工程与生物工程学院, 中国杭州市, 310027
2浙江大学杭州国际科创中心, 中国杭州市, 311215
3上海科技大学物质科学与技术学院, 中国上海市, 201210


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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