Full Text:   <2928>

CLC number: R76

On-line Access: 

Received: 2004-01-16

Revision Accepted: 2004-04-21

Crosschecked: 0000-00-00

Cited: 6

Clicked: 7011

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.10 P.1304-1312


Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection

Author(s):  TANG Jian-guo, LI Xuan, CHEN Ping

Affiliation(s):  Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China

Corresponding email(s):   zhengzheng@mail.hz.zj.cn

Key Words:  Matrix metalloproteinase 9, EB virus, Nasopharyngeal carcinoma

Share this article to: More

TANG Jian-guo, LI Xuan, CHEN Ping. Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection[J]. Journal of Zhejiang University Science A, 2004, 5(10): 1304-1312.

@article{title="Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection",
author="TANG Jian-guo, LI Xuan, CHEN Ping",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection
%A TANG Jian-guo
%A LI Xuan
%A CHEN Ping
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 10
%P 1304-1312
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1304

T1 - Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection
A1 - TANG Jian-guo
A1 - LI Xuan
A1 - CHEN Ping
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 10
SP - 1304
EP - 1312
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.1304

Objective: To evaluate the expression of matrix metalloproteinase-9 (MMP9) in nasopharyngeal carcinoma and the association between MMP9 and Epstein-Barr virus infection. Methods: The MMP9 expression was studied by immunohistochemical analysis; and Epstein-Barr virus encoded small nuclear mRNA-1 (EBER-1) produced by in situ hybridization were examined in 41 nasopharyngeal carcinoma sections, and the relation between them, and the associations of MMP9 with clinical features were statistically analyzed. Results: Positive expression rate of MMP9 was 73.17%. The expression of MMP9 showed significant positive correlation with the expression of EBER-1 (γ=0.483, P=0.001). There was significant association of MMP9 expression with lymph nodes metastasis and clinical stage (P<0.001), non-significant association with age, gender, pathological classification and T classification. Conclusions: The highly pronounced expression of MMP9 is associated with cervical lymph nodes metastasis. Epstein-Barr virus can enhance NPC metastasis by up-regulating the expression of MMP9.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Gaudin, P., Trocme, C., Berthier, S., Kieffer, S., Boutonnat, J., Lamy, C., Suria, A., Garin, J., Morel, F., 2000. TIMP-1/MMP-9 imbalance in an EBV-immortalized B lymphocyte celluar model: evidence for TIMP-1 multifunctional properties. Biochim Biophys Acta., 1499(1-2):19-33.

[2] Horikawa, T., Yoshizaki, T., Sheen, T.S., Lee, S.Y., Furukawa, M., 2000. Association of latent membrane protein 1 and matrix metalloproteinase 9 with metastasis in nasopharyngeal carcinoma. Cancer, 89(4):715-723.

[3] Huang, S., Stupack, D., Liu, A., Cheresh, D., Nemerow, G.R., 2000. Cell growth and matrix invasion of EBV-immortalized human B lymphocytes is regulated by expression of alpha (v) integrins. Oncogene, 19(15):1915-1923.

[4] Lein, M., Jung, K., Ortel, B., Stephan, C., Rothaug, W., Juchem, R., Johannsen, M., Deger, S., Schnorr, D., Loening, S., Krell, H.W., 2002. The new synthetic matrix metalloproteinase inhibitor (Roche 28-2653) reduces tumor growth and prolongs survival in a prostate cancer standard rat model. Oncogene, 21(13):2089-2096.

[5] Liotta, L.A., 1986a. Biochemical interactions of tumor cells with the basement membrane. Ann Rev Biochem, 55:1037.

[6] Liotta, L.A., 1986b. Tumor invasion and metastasis-role of the extracellular matrix, Rhoads memorial award lecture. Cancer Res, 46:1-7.

[7] Liotta, L.A., 1992. Cancer cell invasion and metastasis. Scientific AM, 266(2):34.

[8] Murono, S., Inoue, H., Tanabe, T., Joab, I., Yoshizaki, T., Furukawa, M., Pagano, J.S., 2001. Induction of cyclooxygenase-2 by Epstein-Barr virus latent membrane protein 1 is involved in vascular endothelial growth factor production in nasopharyngeal carcinoma cells. Proc Natl Acad Sci, 98:6905-6910.

[9] Ren, Q., Sato, H., Murono, S., Furukawa, M., Yoshizaki, T., 2004. Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappaB signaling pathway in EBV-infected naso-pharyngeal carcinoma cell line. Laryngoscope, 114(5):855-859.

[10] Takeshita, H., Yoshizaki, T., Miller, W.E., Sato, H., Furukawa, M., Pagano, J.S., Raab-Traub, N., 1999. Matrix metalloproteinase 9 expression is induced by Epstein-Barr virus latent membrane protein 1 C-terminal activation regions 1 and 2. J Virol, 73(7):5548-5555.

[11] Yoshizaki, T., Sato, H., Murono, S., Pagano, J.S., Furukawa, M., 1999. Matrix metalloproteinase 9 is induced by the Epstein-Barr virus BZLF1 transactivator. Clin Exp Metastasis, 17:431-436.

[12] Yoshizaki, T., Horikawa, T., Ren, Q.C., Wakisaka, N., Takeshita, H., Sheen, T.S., Lee, S.Y., Sato, H., Furukawa, M., 2001. Induction of interleukin-8 by epstein-barr virus latent membrane protein-1 and its correlation to angiogenesis in nasopharyngeal carcinoma. Clin Cancer Res, 7:1946-1951.

[13] Zhang, X., Guo, Y., Ye, Q., Yang, Z., Dong, Z., 1999. Study of the relation between MMP2, MMP9 and nasopharyngeal carcinoma. J Clinic Otol, 13(8):356-358.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE