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On-line Access: 2013-07-30

Received: 2013-06-18

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.8 P.696-704

http://doi.org/10.1631/jzus.BQICC703


Effects of rosuvastatin on the production and activation of matrix metalloproteinase-2 and migration of cultured rat vascular smooth muscle cells induced by homocysteine


Author(s):  Ya-fei Shi, Ju-fang Chi, Wei-liang Tang, Fu-kang Xu, Long-bin Liu, Zheng Ji, Hai-tao Lv, Hang-yuan Guo

Affiliation(s):  Department of Cardiology, Shaoxing People’s Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000, China; more

Corresponding email(s):   ghangyuan@hotmail.com

Key Words:  Matrix metalloproteinase-2 (MMP-2), Vascular smooth muscle cells (VSMCs), Migration, Rosuvastatin, Homocysteine


Ya-fei Shi, Ju-fang Chi, Wei-liang Tang, Fu-kang Xu, Long-bin Liu, Zheng Ji, Hai-tao Lv, Hang-yuan Guo. Effects of rosuvastatin on the production and activation of matrix metalloproteinase-2 and migration of cultured rat vascular smooth muscle cells induced by homocysteine[J]. Journal of Zhejiang University Science B, 2013, 14(8): 696-704.

@article{title="Effects of rosuvastatin on the production and activation of matrix metalloproteinase-2 and migration of cultured rat vascular smooth muscle cells induced by homocysteine",
author="Ya-fei Shi, Ju-fang Chi, Wei-liang Tang, Fu-kang Xu, Long-bin Liu, Zheng Ji, Hai-tao Lv, Hang-yuan Guo",
journal="Journal of Zhejiang University Science B",
volume="14",
number="8",
pages="696-704",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.BQICC703"
}

%0 Journal Article
%T Effects of rosuvastatin on the production and activation of matrix metalloproteinase-2 and migration of cultured rat vascular smooth muscle cells induced by homocysteine
%A Ya-fei Shi
%A Ju-fang Chi
%A Wei-liang Tang
%A Fu-kang Xu
%A Long-bin Liu
%A Zheng Ji
%A Hai-tao Lv
%A Hang-yuan Guo
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 8
%P 696-704
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.BQICC703

TY - JOUR
T1 - Effects of rosuvastatin on the production and activation of matrix metalloproteinase-2 and migration of cultured rat vascular smooth muscle cells induced by homocysteine
A1 - Ya-fei Shi
A1 - Ju-fang Chi
A1 - Wei-liang Tang
A1 - Fu-kang Xu
A1 - Long-bin Liu
A1 - Zheng Ji
A1 - Hai-tao Lv
A1 - Hang-yuan Guo
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 8
SP - 696
EP - 704
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.BQICC703


Abstract: 
Objective: To test the influence of homocysteine on the production and activation of matrix metalloproteinase-2 (MMP-2) and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) and on cell migration of cultured rat vascular smooth muscle cells (VSMCs). Also, to explore whether rosuvastatin can alter the abnormal secretion and activation of MMP-2 and TIMP-2 and migration of VSMCs induced by homocysteine. Methods: Rat VSMCs were incubated with different concentrations of homocysteine (50–5000 μmol/L). Western blotting and gelatin zymography were used to investigate the expressions and activities of MMP-2 and TIMP-2 in VSMCs in culture medium when induced with homocysteine for 24, 48, and 72 h. Transwell chambers were employed to test the migratory ability of VSMCs when incubated with homocysteine for 48 h. Different concentrations of rosuvastatin (10−9–10−5 mol/L) were added when VSMCs were induced with 1000 μmol/L homocysteine. The expressions and activities of MMP-2 and TIMP-2 were examined after incubating for 24, 48, and 72 h, and the migration of VSMCs was also examined after incubating for 48 h. Results: homocysteine (50–1000 μmol/L) increased the production and activation of MMP-2 and expression of TIMP-2 in a dose-dependent manner. However, when incubated with 5000 μmol/L homocysteine, the expression of MMP-2 was up-regulated, but its activity was down-regulated. Increased homocysteine-induced production and activation of MMP-2 were reduced by rosuvastatin in a dose-dependent manner whereas secretion of TIMP-2 was not significantly altered by rosuvastatin. homocysteine (50–5000 μmol/L) stimulated the migration of VSMCs in a dose-dependent manner, but this effect was eliminated by rosuvastatin. Conclusions: homocysteine (50–1000 μmol/L) significantly increased the production and activation of MMP-2, the expression of TIMP-2, and the migration of VSMCs in a dose-dependent manner. Additional extracellular rosuvastatin can decrease the excessive expression and activation of MMP-2 and abnormal migration of VSMCs induced by homocysteine.

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