Abstract: Craniofacial muscles represent essential components of the skeletal muscle system, contributing to critical physiological processes. Severe trauma can result in craniofacial volumetric muscle loss (VML), hindering the muscle regenerative process and leading to muscular facial deformities and functional impairment, as well as social isolation and psychological depression. Conventional therapies, involving muscle flap transposition or autologous tissue grafting, only achieve morphological repair but are ineffective in restoring muscle function, often causing complications of donor-area injury and sensory deficit. In this study, we successfully constructed a biomimetic engineered muscle tissue that integrates myofiber alignment, effective innervation, and blood perfusion, which can promote multiple tissue regeneration of the masseter area in vivo, making functional regeneration achievable. Based on light-controlled micropatterning technology, we generated mature muscle fibers with oriented alignment and established a neuromuscular co-culture system for in vitro neuromuscular junction (NMJ) reconstruction. Additionally, we designed and fabricated a vascular network structure to promote the vascularization of tissue, with hydrogel serving as the vehicle for assembling the composite engineered tissue. This technology enables the capability to customize the shape and dimension of the constructed entity to address diverse muscle defects, enabling the goal of personalized repair. In summary, this study provides a promising novel strategy for tissue regeneration that breaks through the current challenges in the treatment of craniofacial VML.