JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

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Bioprinted integrated gradient biomechanical signal-tailored osteosarcoma model: advancing insights into tumor development and drug screening

Author(s): Xuelian Mi, Ya Ren, Hanbo Wang, Lei Qiang, Weiqing Kong, Hui Wang, Xue Yang, Yihao Liu, Han Yang, Di Xiao, Zhenjiang Ma, Guoxing Xu, Changru Zhang, Jinwu Wang
Affiliation(s): Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, 610041 Chengdu, China; more
Corresponding email(s): zcr_ecust@163.com, wangjw-team@shsmu.edu.cn
Key Words: 3D bioprinting, Gradient biomechanical signal-tailor, Osteosarcoma model, Tumor development, Drug screening

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Xuelian Mi, Ya Ren, Hanbo Wang, Lei Qiang, Weiqing Kong, Hui Wang, Xue Yang, Yihao Liu, Han Yang, Di Xiao, Zhenjiang Ma, Guoxing Xu, Changru Zhang, Jinwu Wang. Bioprinted integrated gradient biomechanical signal-tailored osteosarcoma model: advancing insights into tumor development and drug screening[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/bdm.2400108

@article{title="Bioprinted integrated gradient biomechanical signal-tailored osteosarcoma model: advancing insights into tumor development and drug screening",
author="Xuelian Mi, Ya Ren, Hanbo Wang, Lei Qiang, Weiqing Kong, Hui Wang, Xue Yang, Yihao Liu, Han Yang, Di Xiao, Zhenjiang Ma, Guoxing Xu, Changru Zhang, Jinwu Wang",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/bdm.2400108"
}

%0 Journal Article
%T Bioprinted integrated gradient biomechanical signal-tailored osteosarcoma model: advancing insights into tumor development and drug screening
%A Xuelian Mi
%A Ya Ren
%A Hanbo Wang
%A Lei Qiang
%A Weiqing Kong
%A Hui Wang
%A Xue Yang
%A Yihao Liu
%A Han Yang
%A Di Xiao
%A Zhenjiang Ma
%A Guoxing Xu
%A Changru Zhang
%A Jinwu Wang
%J Journal of Zhejiang University SCIENCE B
%P
%@ 2095-9184
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/bdm.2400108"

TY - JOUR
T1 - Bioprinted integrated gradient biomechanical signal-tailored osteosarcoma model: advancing insights into tumor development and drug screening
A1 - Xuelian Mi
A1 - Ya Ren
A1 - Hanbo Wang
A1 - Lei Qiang
A1 - Weiqing Kong
A1 - Hui Wang
A1 - Xue Yang
A1 - Yihao Liu
A1 - Han Yang
A1 - Di Xiao
A1 - Zhenjiang Ma
A1 - Guoxing Xu
A1 - Changru Zhang
A1 - Jinwu Wang
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 2095-9184
Y1 - in press
PB - Zhejiang University Press & Springer
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doi="https://doi.org/10.1631/bdm.2400108"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: In current osteosarcoma research and drug screening, in vitro 3D models are favored for overcoming the limitations of traditional models. In in vitro 3D models, tumor microenvironment simulation, especially mechanical microenvironment, is crucial for tumor biological effects. However, current in vitro tumor model construction for osteosarcoma is often limited to a single mechanical signal, which fails to simulate the diversity of osteosarcoma mechanical stimuli. In this study, we utilized embedded bioprinting technology and multiple response properties of calcium ions in soft stiffness systems, hard stiffness systems, and osteosarcoma cell biological functions to construct an integrated gradient biomechanical signal-tailored osteosarcoma model. Specifically, we achieved this by printing a fibrinogen bioink containing calcium ions and osteosarcoma tumor spheroids within an extracellular matrix composed of methacryloylated alginate, methacryloylated gelatin, and thrombin, which is rich in polysaccharides and proteins and exhibits self-healing properties. In vitro and in vivo studies showed that the integrated gradient biomechanical signal-tailored osteosarcoma model enhanced tumor stemness, proliferation and migration, successfully reproduced the nest-like structure of cancer, and provided an in vitro research platform closer to a natural tumor. In conclusion, this study proposes a novel integrated gradient biomechanical signal-tailored osteosarcoma model construction system that provides a new strategy for the clinical understanding of tumor development, drug screening, and exploration of drug resistance and metastasis mechanisms.

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